Whole spectrum fluorescence detection with ultrafast white light excitation
AssigneeThe Regents of the University of Michigan
InventorsJing Ye, Theodore Norris, James Baker
Life of this patent
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Abstract
A fluorescence detection system for testing a sample having at least one fluorophore. The fluorescence detection system comprises a white light generation system outputting a white light pulse. The white light pulse has a first frequency range and a first time duration. The white light pulse excites the at least one fluorophore of the sample to emit a fluorescence. The fluorescence has a second frequency range and a second time duration, wherein the first time duration is less than the second time duration. A time-resolving detector receives the fluorescence and at least a portion of the white light pulse and separates the fluorescence from the portion of the white light pulse.
Claims26
A fluorescence detection system for testing a sample, said sample having at least one fluorophore, said fluorescence detection system comprising:
- a white light generation system outputting a white light pulse, said white light pulse having a first frequency range and a first time duration, said white light pulse exciting the at least one fluorophore of the sample to emit a fluorescence, said fluorescence having a second frequency range and a second time duration, said first time duration being less than said second time duration;
- and
- a time-resolving detector receiving said fluorescence and at least a portion of said white light pulse, said time-resolving detector separating said fluorescence from said portion of said white light pulse.
A fluorescence detection system for testing a sample, said sample having at least one fluorophore, said fluorescence detection system comprising:
- a laser source outputting a laser pulse having a first time duration;
- a member having a non-linear refractive index, said member receiving said laser pulse therethrough and outputting a white light pulse, said white light pulse having a first frequency range and a second time duration, said white light pulse exciting the at least one fluorophore of the sample to emit a fluorescence, said fluorescence having a second frequency range and a third time duration, said second time duration being less than said third time duration;
- and
- a time-resolving detector receiving said fluorescence and at least a portion of said white light pulse, said time-resolving detector separating said fluorescence from said portion of said white light pulse.
A fluorescence detection system for testing a sample, said sample having a first fluorophore and a second fluorophore, said fluorescence detection system comprising:
- a white light generation system outputting a white light pulse, said white light pulse having a first frequency range and a first time duration, said white light pulse exciting the first fluorophore and the second fluorophore to emit a first fluorescence and a second fluorescence respectively, said first fluorescence having a second frequency range that is different than a third frequency range of said second fluorescence, said first fluorescence having a second time duration and said second fluorescence having a third time duration, said first time duration being less than said second time duration and said third time duration;
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- a time-resolving detector receiving said first fluorescence, said second fluorescence, and at least a portion of said white light pulse, said time-resolving detector separating said first fluorescence and said second fluorescence from said portion of said white light pulse.
Dependencies are inferred from the claim text.