Arrangement for optimizing the pulse shape in a laser scanning microscope
AssigneeCarl Zeiss MicroImaging GmbH
InventorsRalf Wolleschensky, Thomas Feurer, Roland Sauerbrey
Life of this patent
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Abstract
A Device for coupling a short pulse laser into a microscope beam path, wherein the spectral components of the laser radiation are spatially separated by means of a dispersive element, the individual spectral components are manipulated and are then spatially superimposed again by means of another dispersive element.
Claims22
A device for coupling a short pulse laser into a microscope beam path of a laser scanning microscope used for investigation of nonlinear contrast methods comprising:
- a dispersive element for spatially separating the spectral components of the laser radiation;
- means for manipulating individual spectral components;
- said manipulating means acts to manipulate components and the manipulator means is purposefully optimized by feeding back a measurement signal and the desired measurement signal is therefore adjusted;
- and an element for spatially superimposing the manipulated individual spectral components.
The device according to claim 21, wherein the phase modulation in the manipulator means is optimized depending on the center wavelength of the short pulse laser by the use of feedback.
The device according to claim 21, wherein the phase modulation in the manipulator means is optimized depending on the utilized objective by the use of the feedback.
The device according to claim 21, wherein the phase modulation in the manipulator means is optimized depending on the utilized average output by the use of feedback.
The device according to claim 21, wherein, by the use of feedback, the phase modulation in the manipulator means is adjusted depending on the depth of penetration into a preparation to be examined and a nonlinearly excited fluorescence signal is therefore optimized.
The device according to claim 21, wherein a pulse front and a spherical aberration are optimized additionally by an adaptive acousto-optic element.
The device according to claim 21, wherein the phase modulation in the manipulator means is optimized depending on the utilized objective by the use of feedback.
The device according to claim 21, wherein a specific excitation of fluorescence dyes is carried out by phase modulation and amplitude modulation in the manipulator means.
A device for coupling a short pulse laser into a microscope beam path of a laser scanning microscope comprising:
- a dispersive element for spatially separating the spectral components of the laser radiation;
- means for manipulating individual spectral components;
- wherein the manipulator means is purposefully optimized by feeding back a measurement signal and the desired measurement signal is therefore adjusted;
- and
- an element for spatially superimposing the manipulated individual spectral components.
A laser scanning microscope system comprising:
- a microscope
- a dispersive element for spatially separating the spectral components of a short pulse laser that will be coupled into a microscope beam path of said microscope means for manipulating individual spectral components;
- wherein the manipulating means is purposefully optimized by feeding back a measurement signal and the desired measurement signal is therefore adjusted;
- and an element for spatially superimposing the manipulated individual spectral components.
Dependencies are inferred from the claim text.