Method for improving depth discrimination in optical reproduction systems
AssigneeCarl Zeiss MicroImaging GmbH
InventorsBernhard Backs, Joerg Schaffer, Lutz Schaefer, Martin Voelcker
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Abstract
The invention is directed to a method for improving the depth discrimination in optically imaging systems. It is applicable in particular in light microscopy for improving image quality when examining three-dimensionally extending objects. It is applicable in the method of structured illumination as described in WO 97/6509. For this purpose, influences due to variations in the brightness of the light source, positioning of the imaged periodic structure and bleaching of the object in fluorescence illumination are determined and taken into account in the calculation of the object structure.
Claims14
1-13. (canceled)
A method for improving the depth discrimination of optical systems comprising the steps of:
- a) illuminating an object with a periodic structure;
- b) registering the resulting brightness distribution;
- c) creating displacement of the phase position of the periodic structure;
- d) repeating steps a) to c) until at least three brightness distributions have been registered;
- e) calculating the registered brightness distributions to obtain an object brightness distribution;
- f) registering the displacements of the phase positions from c);
- g) detecting brightness variations of the illumination;
- h) determining bleaching phenomena of the object in fluorescence illumination;
- and
- i) carrying out calculation taking into account the results obtained in steps f) to h).
Dependencies are inferred from the claim text.