Methods and means for increasing the tolerance of plants to stress conditions
AssigneeBayer BioScience N.V.
InventorMarc De Block
Life of this patent
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Abstract
Methods and means are provided to increase the tolerance of plants to abiotic stress or adverse growing conditions, including drought, high light intensities, high temperatures, nutrient limitations and the like by reducing the activity of endogenous PARG proteins in plants.
Claims19
A method of producing a plant tolerant to stress conditions comprising the steps of
- (a) providing plant cells with a chimeric gene to create transgenic plant cells, said chimeric gene comprising the following operably linked DNA fragments
- (i) a plant-expressible promoter;
- (ii) a DNA region, which when transcribed yields an ParG inhibitory RNA molecule;
- (iii) a 3′ end region involved in transcription termination and polyadenylation;
- (b) regenerating a population of transgenic plant lines from said transgenic plant cell;
- and
- (c) identifying a stress tolerant plant line within said population of transgenic plant lines.
A method of producing a plant tolerant to stress conditions comprising the steps of:
- (a) isolating a DNA fragment of at least 100 bp comprising a part of the parG encoding gene of said plant;
- (b) producing a chimeric gene by operably linking the following DNA fragments;
- (i) a plant expressible promoter region;
- (ii) said isolated DNA fragment comprising part of the parG encoding gene of said plant in direct orientation compared to the promoter region;
- (iii) said isolated DNA fragment comprising part of the parG encoding gene of said plant in inverted orientation compared to the promoter region;
- (iv) a 3′ end region involved in transcription termination and polyadenylation;
- (c) providing plant cells with said chimeric gene to create transgenic plant cells
- (d) regenerating a population of transgenic plant lines from said transgenic plant cell;
- and
- (e) identifying a stress tolerant plant line within said population of transgenic plant lines.
A DNA molecule comprising
- (i) a plant-expressible promoter;
- (ii) a DNA region, which when transcribed yields a ParG inhibitory RNA molecule;
- and
- (iii) a 3′ end region involved in transcription termination and polyadenylation.
A method of producing a plant tolerant to stress conditions comprising the steps of
- (a) providing plant cells with a chimeric gene to create transgenic plant cells, said chimeric gene comprising the following operably linked DNA fragments
- (i) a plant-expressible promoter;
- (ii) a DNA region, which when transcribed yields an ParG inhibitory RNA molecule, said DNA region comprising a nucleotide sequence of at least 21 to 100 nucleotides of a nucleotide sequence encoding a protein comprising the amino acid sequence of SEQ ID No 1, 2 or 16 or at least 21 to 100 nucleotides of a nucleotide sequence of SEQ ID 3, 4, 15 or 23;
- (iii) a 3′ end region involved in transcription termination and polyadenylation;
- (b) regenerating a population of transgenic plant lines from said transgenic plant cell;
- and
- (c) identifying a stress tolerant plant line within said population of transgenic plant lines.
A method of producing a plant tolerant to stress conditions comprising the steps of
- (a) subjecting a plant cell line or a plant or plant line, to mutagenesis;
- (b) identifying those plant cells or plants that have a mutation in an endogenous ParG gene;
- (c) subjecting the identified plant cells or plants to stress conditions;
- (d) identifying plant cells or plants that tolerate said stress conditions better than control plants.
A method of producing a plant tolerant to stress conditions comprising the steps of
- (a) selecting a plant cell line or a plant or plant line which is resistant to a ParG inhibitor;
- (b) identifying those plant cells or plants that have a mutation in an endogenous ParG gene;
- (c) subjecting the identified plant cells or plants to stress conditions;
- (d) identifying plant cells or plants that tolerate said stress conditions better than control plants.
A stress tolerant plant cell or plant comprising a mutation in an endogenous ParG gene.
Dependencies are inferred from the claim text.