Novel assay to screen for anti-malarials
AssigneeIndian Institute of Science
InventorsTatu Utpal, Pavithra Soundara Raghavan, Banumathy Gowrishankar
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Abstract
Malarial parasite Plasmodium falciparum is responsible for the most severe form of malaria in humans, causing about 2 million deaths every year. Lack of effective vaccines and emergence of drug-resistant strains necessitate the need of novel drug targets to treat the disease. The present invention describes a novel assay method of identifying candidate compounds as anti-malarials based on the property of binding to plasmodial parasite 90 kDa heat shock protein.
Claims6
A novel assay to screen for anti-malarial drugs by testing for the binding of the test compound with plasmodium 90 kDa heat shock protein which comprises:
- a) Covalent immobilization of the test compound on suitable matrices such as Sepharose or BIAcore CM5 sensor chips by known methods;
- b) Preparation of saponin-freed Plasmodial trophozoite lysate by known methods;
- c) Reacting the trophozoite lysate with the covalently immobilized test compound, and
- d) Detection of the compound bound Plasmodial 90 kDa heat shock protein by known methods.
A novel assay as claimed in any one of the preceding claims, wherein for compounds of unknown structure, derivatization is done with biotin using photobiotin acetate followed by BIAcore analysis using the BIAcore SA chip that carries a streptavidin surface.
Dependencies are inferred from the claim text.