Method for selecting cell lines to be used for nuclear transfer in mammalian species
AssigneeGTC Biotherapeutics, Inc.
InventorsDavid Melican, Robin Butler, William Gavin
Life of this patent
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Abstract
The present invention provides data to demonstrate that the fusion performance of a cell-line in procedures involving fusion and cleavage indices either alone or in combination are a means for selecting a cell lines that will be successful in a nuclear transfer or microinjection program. This technique and method of selecting a cell line offers an additional alternative and improvement in the creation of activated and fused nuclear transfer-capable embryos for the production of live offspring in various mammalian non-human species including goats, pigs, rodents, primates, rabbits and cattle.
Claims26
1-23. (canceled)
A method for producing cultured inner cell mass cells, comprising:
- (i) obtaining desired differentiated mammalian cells to be used as a source of donor nuclei;
- (ii) obtaining at least one oocyte from a mammal of the same species as the cells which are the source of donor nuclei;
- (iii) enucleating said at least one oocyte;
- (iv) transferring the desired differentiated cell or cell nucleus into the enucleated oocyte;
- (v) simultaneously fusing and activating the cell couplet to form a first transgenic embryo;
- (vi) activating a cell-couplet to create a first transgenic embryo that is activated after an initial electrical shock;
- and
- (vi) culturing cells obtained from said cultured activated embryo to obtain cultured inner cell mass cells;
- (vii) wherein the desired differentiated mammalian cell line to be used as a karyoplast is selected according to the objective parameters of cleavage and/or fusion patterns
48-53. (canceled)
Dependencies are inferred from the claim text.