Antithrombosis enzyme from the snake venom of Agkistrodon acutus
OwnerChun Wang, Benjamin Li +11inventors
InventorsChun Wang, Benjamin Li, Xin Cheng, Jing Liu, Li-Wen Niu, Wan-Zhi Huang, Zhen-Yu Xu, Dan Luo, Lian-Di Kang, Jin-Guo Ding, Fang Rong, Yan Liu, Hui-Ran Chen
Life of this patent
Estimating the term…
Abstract
This invention features an antithrombosis enzyme extracted and purified from the snake venom of Southern-Anhui Agkistrodon acutus and pharmaceutical uses thereof.
Claims22
An isolated, purified or recombinant antithrombosis enzyme having the following characteristics:
- the molecular weight of said enzyme is between about 28 kD and about 32 kD when analyzed by polyacrylamide gel electrophoresis,
- the aspartic acid content of said enzyme is between about 2% and about 5%,
- the glutamic acid content of said enzyme is between about 2% and about 5%, and
- said enzyme hydrolyzes fibrin, dissolves blood clots, and prevents platelet aggregation.
An isolated, purified or recombinant polypeptide comprising no less than 20 contiguous amino acids from SEQ ID NO:
- 2.
An isolated, purified or enriched recombinant nucleic acid comprising no less than 60 contiguous nucleotides from SEQ ID NO:
- 1 or its fully complementary strand of the same length and a promoter effective to initiate transcription of said contiguous nucleotides in a host cell.
An isolated, purified, or recombinant polypeptide comprising SEQ ID NO:
- 2.
An isolated, purified or enriched recombinant nucleic acid comprising a contiguous nucleic acid sequence encoding SEQ ID NO:
- 2 and a promoter effective to initiate transcription of said contiguous nucleic acid sequence in a host cell.
An isolated, purified or enriched recombinant nucleic acid comprising SEQ ID NO:
- 1 or its fully complementary strand of the same length and a promoter effective to initiate transcription of said contiguous nucleotides in a host cell.
Dependencies are inferred from the claim text.