# US 2006/0150896 A1 · Labware for high-throughput free-interface diffusion crystallization > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2006/0150896 A1 - Document ID: US20060150896A1 - Status: Published application - Kind: A1 - Application number: 11/172,685 - Filed: 2005-06-30 - Published: 2006-07-13 - Earliest claimed: 2005-01-10 - Assignees: The Regents of the University of California - Inventors: Brent Segelke; Timothy Lekin; Dominique Toppani - Web page: https://patentgrep.com/patent/US20060150896A1 ## CPC - C30B 29/58: Macromolecular compounds - C30B 7/00: Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions (from molten solvents C30B9/00; by normal or gradient freezing C30B11/00; under a protective fluid C30B27/00) - Y10T 117/1024: for crystallization from liquid or supercritical state - C30B: SINGLE-CRYSTAL GROWTH (by using ultra-high pressure, e.g. for the formation of diamonds, B01J3/06); UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL (zone-refining of metals or alloys C22B); PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE (casting of metals, casting of other substances by the same processes or devices B22D; working of plastics B29; modifying the physical structure of metals or alloys C21D, C22F); SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE (for producing semiconductor devices or parts thereof H10); APPARATUS THEREFOR - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract A system for crystallization of a protein sample comprising a first mixing cell, a reagent cocktail that is adapted to be added to the first mixing cell, a second mixing cell with the protein sample adapted to be added to the second mixing cell, and a transfer device between the first mixing cell and the second mixing cell. The transfer device comprises structure that allows the reagent cocktail in the first mixing cell to flow into the second mixing cell and mix with the protein sample and allows the protein sample in the second mixing cell to flow into the first mixing cell and mix with the reagent cocktail. The mixture of the reagent cocktail and the protein sample are allowed to incubate to produce protein crystals. ## Claims ### Claim 1 A system for crystallization of a protein sample, comprising: a first mixing cell, a reagent cocktail, said reagent cocktail adapted to be added to said first mixing cell, a second mixing cell, the protein sample adapted to be added to said second mixing cell, and a transfer device between said first mixing cell and said second mixing cell. ### Claim 2 (depends on 1) The system for crystallization of claim 1 wherein said transfer device comprises structure that allows said reagent cocktail in said first mixing cell to flow into said second mixing cell and mix with the protein sample and allows the protein sample in said second mixing cell to flow into said first mixing cell and mix with said reagent cocktail. ### Claim 3 (depends on 1) The system for crystallization of claim 1 wherein said transfer device comprises means for allowing said reagent cocktail in said first mixing cell to flow into said second mixing cell and mix with the protein sample and allowing the protein sample in said second mixing cell to flow into said first mixing cell and mix with said reagent cocktail. ### Claim 4 (depends on 1) The system for crystallization of claim 1 wherein said transfer device comprises a water permeable plug or a channel or a bridge or a membrane or a capillary bridge or a gel filled capillary bridge that allows said reagent cocktail in said first mixing cell to flow into said second mixing cell and mix with the protein sample and allows the protein sample in said second mixing cell to flow into said first mixing cell and mix with said reagent cocktail. ### Claim 5 (depends on 1) The system for crystallization of claim 1 wherein said transfer device is a water permeable plug. ### Claim 6 (depends on 1) The system for crystallization of claim 1 wherein said transfer device is a channel. ### Claim 7 (depends on 1) The system for crystallization of claim 1 wherein said transfer device is a membrane. ### Claim 8 (depends on 1) The system for crystallization of claim 1 wherein said transfer device is a capillary bridge. ### Claim 9 (depends on 1) The system for crystallization of claim 1 wherein said transfer device is a gel filled capillary bridge. ### Claim 10 (depends on 1) The system for crystallization of claim 1 wherein said first mixing cell has a parallelogram shape. ### Claim 11 (depends on 1) The system for crystallization of claim 1 wherein said first mixing cell and said second mixing cell have a parallelogram shape. ### Claim 12 (depends on 1) The system for crystallization of claim 1 wherein said first mixing cell is cylindrical. ### Claim 13 (depends on 1) The system for crystallization of claim 1 wherein said first mixing cell and said second mixing cell are cylindrical. ### Claim 14 (depends on 1) The system for crystallization of claim 1 including a reservoir connected to said first mixing cell and said second mixing cell. ### Claim 15 (depends on 1) The system for crystallization of claim 1 including a reservoir connected to said first mixing cell with said reagent cocktail in said reservoir. ### Claim 16 (depends on 1) The system for crystallization of claim 1 including a chamber plate with said first mixing cell, said second mixing cell, and said transfer device connected to said chamber plate. ### Claim 17 (depends on 1) The system for crystallization of claim 1 including a multi-chamber plate with said first mixing cell, said second mixing cell, and said transfer device connected to said multi-chamber plate and including at least one reagent mixing cell, at least one sample mixing cell, and at least one transfer device connected to said multi-chamber plate. ### Claim 18 (depends on 1) The system for crystallization of claim 1 including a ninety six chamber plate with said first mixing cell, said second mixing cell, and said transfer device connected to said ninety six chamber plate and including additional reagent mixing cells, additional sample mixing cells, and additional transfer devices make up said ninety six chamber plate. ### Claim 19 A method of crystallization for producing protein crystals, comprising: providing a first mixing cell, providing a second mixing cell, providing a transfer device between said first mixing cell and said second mixing cell, adding a reagent cocktail to said first mixing cell, adding the protein sample to said second mixing cell, allowing said reagent cocktail and the protein sample to mix through said transfer device creating a mixture of said reagent cocktail and the protein sample, and incubating said mixture of said reagent cocktail and the protein sample to produce the protein crystals. ### Claim 20 (depends on 19) The method of crystallization of claim 19 wherein said step of adding a reagent cocktail to said first mixing cell comprises adding a reagent cocktail, previously derived to said first mixing cell. ### Claim 21 (depends on 19) The method of crystallization of claim 19 including the steps of providing a reservoir connected to said first mixing cell and adding said reagent cocktail to said reservoir.