Gene suppression in transgenic plants using multiple constructs

A method of gene suppression comprising transforming eukaryotic cells with multiple copies of gene suppression cassettes, wherein said method comprises

• (a) assembling a DNA construct comprising a first gene suppression cassette adjacent to a second gene suppression cassette, wherein said first cassette comprises a first promoter operably linked to DNA of a gene targeted for suppression in either a sense or an anti-sense orientation, wherein said second cassette comprises a second promoter operably linked to at least a part of said DNA in the same orientation, wherein said first and second cassettes are assembled so that the promoter elements are at the ends of the construct,
• (b) transforming said eukaryotic cells by transferring an assembly of said first and second cassettes into said cells,
• (c) regenerating a transgenic organism from cells transformed with said assembly, and
• (d) whereby a trait resulting from suppression of the level of protein encoded by said DNA of a gene targeted for suppression can be observed in said organism.