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US 2006/0150285 A1Published application
Nucleotide sequences and polypeptides encoded thereby for enhancing plant drought tolerance
AssigneeCeres, Inc.
InventorsGregory Nadzan, Kenneth Feldmann, Cory Christensen
Life of this patent
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Abstract
Isolated polynucleotides and polypeptides encoded thereby are described, together with the use of those products for making transgenic plants with increased tolerance to abiotic stress (e.g., high or low temperature, drought, flood).
Claims25
An isolated nucleic acid molecule comprising:
- (a) a nucleotide sequence encoding an amino acid sequence that is at least 85% identical to any one of Leads 68, 69, 94 and 95, corresponding to SEQ ID Nos. ______-______, respectively;
- (b) a nucleotide sequence that is complementary to any one of the nucleotide sequences according to paragraph (a);
- (c) a nucleotide sequence according to any one of SEQ ID Nos. ______-______;
- (d) a nucleotide sequence that is in reverse order of any one of the nucleotide sequences according to (c) when read in the 5′ to 3′ direction;
- (e) a nucleotide sequence that is an interfering RNA to the nucleotide sequence according to paragraph (a);
- (f) a nucleotide sequence able to form a hybridized nucleic acid duplex with the nucleic acid according to any one of paragraphs (a)-(d) at a temperature from about 40° C. to about 48° C. below a melting temperature of the hybridized nucleic acid duplex;
- (f) a nucleotide sequence encoding any one of the amino acid sequences identified as Leads 68, 69, 94 and 95, corresponding to SEQ ID Nos. ______, respectively;
- or
- (g) a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in FIGS. 1-4 or one of the polypeptide sequences in SEQ ID NOs. 1-93 and 173-176.
6 more elements
A method for increasing drought tolerance in a plant, said method comprising introducing into a plant cell an isolated nucleic acid comprising:
- (a) a nucleotide sequence encoding an amino acid sequence that is at least 85% identical to any one of Leads 68, 69, 94 and 95, corresponding to SEQ ID Nos. ______-______, respectively;
- (b) a nucleotide sequence that is complementary to any one of the nucleotide sequences according to paragraph (a);
- (c) a nucleotide sequence according to any one of SEQ ID Nos. ______-______;
- (d) a nucleotide sequence that is in reverse order of any one of the nucleotide sequences according to (c) when read in the 5′ to 3′ direction;
- (e) a nucleotide sequence that is an interfering RNA to the nucleotide sequence according to paragraph (a);
- (f) a nucleotide sequence able to form a hybridized nucleic acid duplex with the nucleic acid according to any one of paragraphs (a)-(d) at a temperature from about 40° C. to about 48° C. below a melting temperature of the hybridized nucleic acid duplex;
- (f) a nucleotide sequence encoding any one of the amino acid sequences identified as Leads 68, 69, 94 and 95, corresponding to the full length or the CDS identified in SEQ ID Nos. 6, 25, 57 and 71, respectively;
- or
- (g) a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in FIGS. 1-4 or one of the polypeptide sequences in SEQ ID NOs. 1-93 and 173-176, wherein said plant produced from said plant cell has increased drought tolerance as compared to the corresponding level of a control plant that does not comprise said nucleic acid.
6 more elements
A plant cell comprising an isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding an amino acid sequence that is at least 85% identical to any one of Leads 68, 69, 94 and 95, corresponding to SEQ ID Nos. ______-______, respectively;
- (b) a nucleotide sequence that is complementary to any one of the nucleotide sequences according to paragraph (a);
- (c) a nucleotide sequence according to the full length sequence or CDS of any one of SEQ ID Nos. 6, 25, 57 and 71;
- (d) a nucleotide sequence that is in reverse order of any one of the nucleotide sequences according to (c) when read in the 5′ to 3′ direction;
- (e) a nucleotide sequence that is an interfering RNA to the nucleotide sequence according to paragraph (a);
- (f) a nucleotide sequence able to form a hybridized nucleic acid duplex with the nucleic acid according to any one of paragraphs (a)-(d) at a temperature from about 40° C. to about 48° C. below a melting temperature of the hybridized nucleic acid duplex;
- (f) a nucleotide sequence encoding any one of the amino acid sequences identified as Leads 68, 69, 94 and 95, corresponding to SEQ ID Nos. ______-______, respectively;
- or
- (g) a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in FIGS. 1-4.
6 more elements
A method for increasing drought tolerance in a plant, comprising:
- (a) transforming a plant with a nucleic acid molecule comprising a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in any one of FIGS. 1-4;
- and
- (b) expressing said nucleotide sequence in said transformed plant, whereby said transformed plant has an increased drought tolerance as compared to a plant that has not been transformed with said nucleotide sequence.
Dependencies are inferred from the claim text.