Bioremediation with transgenic plants
OwnerAlexander Sorokin, Galina Brychkova +2inventors
InventorsAlexander Sorokin, Galina Brychkova, Nikolai Kartel, Jonathan Jones
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Abstract
Methods are provided for phytoremediating environments contaminated with at least one heavy metal or oil hydrocarbon (or both), the methods comprising use of transgenic plants which express one or more enzymes having rhamnosyltransferase activity (e.g. encoding the rh1A and rh1B genes derived from Pseudomonas aeruginosa). Also provided are related methods, materials and processes for producing and using such transgenic plants.
Claims32
A method of phytoremediating an environment which is contaminated with at least one heavy metal or oil hydrocarbon, which method comprises:
- (a) providing a transgenic plant, which plant expresses at least one heterologous nucleic acid encoding an enzyme having rhamnosyltransferase activity,
- (b) planting or locating said transgenic plant in said environment.
A method of producing a transgenic plant having improved phytoremediating properties with respect to heavy metal or oil hydrocarbon pollutants, the method comprising:
- (i) introducing into a plant cell a heterologous nucleic acid vector encoding at least one enzyme having rhamnosyltransferase activity,
- (ii) causing or allowing recombination between the nucleic acid vector and the plant cell genome to introduce the nucleotide sequence encoding the enzyme having rhamnosyltransferase activity into the plant genome,
- (iii) regenerating the transformed plant cell into a plant, such that the or each enzyme is expressed in the plant.
A recombinant plant vector which comprises a nucleotide sequence encoding at least one enzyme having rhamnosyltransferase activity.
(canceled)
Dependencies are inferred from the claim text.