Method for generating maize plants with an increased leaf starch content, and their use for making maize silage
AssigneePlantTec Biotechnologie GmbH Forschung & Entwicklung
InventorsClaus Frohberg, Michael Bauerlein
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Abstract
There is described a method for generating transgenic maize plants with an increased leaf starch content. Such plants are suitable for making an improved silage. There are furthermore described the use of the transgenic maize plants for making silage, and suitable ensiling methods using such plants.
Claims25
A method for generating a transgenic maize plant with an increased starch content in comparison to corresponding wild-type plants, where
- (a) a cell of a maize plant is genetically modified by introducing a foreign nucleic acid molecule whose presence or expression leads to a reduced activity of an R1 protein which occurs endogenously in the plant cell;
- (b) a plant is regenerated from the cell generated in step (a);
- (c) if appropriate, further plants are generated starting from the plant generated in step (b);
- and
- (d) the foreign nucleic acid molecule according to step (a) is a DNA molecule which encodes at least one antisense RNA which brings about reduced expression of endogenous genes which encode R1 proteins.
A method for making a silage, comprising the step of ensiling transgenic maize plants with a reduced activity of an endogenous R1 protein where the transgenic maize plants were generated by the following steps:
- (a) a cell of a maize plant is genetically modified by introducing a foreign nucleic acid molecule whose presence or expression leads to a reduced activity of an R1 protein which occurs endogenously in the plant cell;
- (b) a plant is regenerated from the cell generated in step (a);
- (c) if appropriate, further plants are generated starting from the plant generated in step (b);
- and
- (d) the foreign nucleic acid molecule according to step (a) is a DNA molecule which encodes at least one antisense RNA which brings about reduced expression of endogenous genes which encode R1 proteins.
A method for making silage which comprises ensuing transgenic maize plants with a reduced activity of an endogenous R1 protein where the reduced activity is caused by a DNA molecule which encodes at least one antisense RNA.
Silage which is produced by the process which comprises silage ensiling transgenic maize plants with a reduced activity of an endogenous R1 protein where the reduced activity is caused by a DNA molecule which encodes at least one antisense RNA.
Dependencies are inferred from the claim text.