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OwnerCatherine Martin, Anthony Michael +1inventors
InventorsCatherine Martin, Anthony Michael, Ricarda Niggeweg
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The invention discloses methods for controlling chlorogenic acid synthesis by manipulation of hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) genes. Isolated nucleic acids encoding HQT and methods for their use are provided. Preferred embodiments are the nucleotide sequences which encode the polypeptide sequences of FIG. 3 (sequences of FIG. 7). Also provided are variant sequences (e.g. alleles and orthologues) and complementary sequences, plus vectors, host cells and plants. Methods of the invention include the use of nucleic acids to express or down-regulate HQT in plant cells and plants. The methods may be used to alter one or more characteristics in a plant e.g. texture, flavour and antioxidant properties.
An isolated nucleic acid which comprises a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) nucleotide sequence encoding a polypeptide which has HQT activity.
A recombinant vector which comprises an HQT nucleic acid said nucleic acid being selected from the group consisting of SEQ ID NO:
A method for influencing chlorogenic acid levels in a plant, which method comprises the step of causing or allowing expression of a heterologous nucleic acid within the cells of the plant, which nucleic acid comprises a HQT nucleotide sequence or the complement thereof, following an earlier step of introducing the nucleic acid into a cell of the plant or an ancestor thereof.
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