Intracellular antibodies for a retrovirus protein
AssigneeErasmus University
InventorsDubravka Drabek, Sylvia Dekker, Franklin Grosveld
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Abstract
A transgenic organism is provided comprising a polynucleotide construct encoding an intracellular antibody which disrupts the catalysis of the production of the xenoantigen galactose α 1,3 galactose and/or a polynucleotide construct which encodes an intracellular antibody which binds specifically to a retrovirus protein, such as a PERV particle protein. Also described are methods for the production of such organisms. Cells, tissues and organs of the transgenic organism may be used in xenotransplantation.
Claims37
A non-human transgenic organism comprising a polynucleotide construct encoding an intracellular immunoglobulin.
A non-human transgenic organism comprising a polynucleotide construct encoding an intracellular immunoglobulin which binds specifically to a retroviral protein.
The non-human transgenic organism according to any one of the preceding claims wherein said single chain antibody is a llama single domain antibody (VHH) having an amino acid sequence of anyone of the sequences set out in FIG. 2.
A method of producing a non-human transgenic organism, said method comprising inserting into the genome of said organism a polynucleotide construct encoding an immunoglobulin which binds specifically to an enzyme which catalyses the production of galactose α-1,3-galactose.
A method of producing a non-human transgenic organism, said method comprising inserting into the genome of said organism a polynucleotide construct encoding an intracellular immunoglobulin which binds specifically to a retroviral protein.
A polynucleotide construct encoding an intracellular immunoglobulin which binds specifically to an enzyme which catalyses the production of galactose α-1,3-galactose.
A polynucleotide construct encoding an intracellular immunoglobulin which binds specifically to a retroviral protein.
A polynucleotide encoding a gag binding protein having an amino acid sequence as set out in FIG. 2.
An immunoglobulin having an amino acid sequence of any of the polypeptides set out in FIG. 2.
Dependencies are inferred from the claim text.