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AssigneeProligo LLC
InventorKhalil Arar
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The present invention relates to novel methods and novel solid support materials for the tandem synthesis of two or more different oligonucleotides on the same solid support in one synthetic run. The methods involve novel support preparations comprised of two or more types of orthogonally protected anchor groups. Subsequent to the selective removal of the first of the respective protective groups, the first oligonucleotide is assembled on the deblocked anchor groups according to standard methods, preferably via phosphoramidite chemistry. Following the capping of said first oligonucleotide, the anchor groups blocked by the second type of protective group are selectively liberated and serve is the starting point for the assembly of a second oligonucleotide, and so forth. After completion of all of the syntheses on the solid support, the oligonucleotides are released from the solid support and deprotected at the nucleobases, using standard methods. Preparations obtained using the method of this invention, generally contain two or more different oligonucleotides. Such preparations are particularly useful in applications that require pairs of oligonucleotide primers, several probes at a time, duplexed nucleic acid fragments, or other combinations of oligonucleotides that are useful in applications such as PCR, sequencing, multiplexed genotyping, cloning and RNA interference. The invention includes procedures for the preparation of the novel solid supports of the invention.
A method for the synthesis of two or more different oligonucleotides comprising the steps of:
A method for the synthesis of two or more different oligonucleotides comprising the steps of:
A method for preparing a homogeneous solid support comprising the steps:
A method for preparing a homogeneous solid support comprising the steps:
A preloaded homogeneous solid support comprised of two or more independently selected linker subsets, wherein each linker subset is comprised of an independently selected nucleosidic moiety, wherein each nucleosidic moiety is comprised of anchor groups that are protected by different protective groups that are orthogonal to each other.
A universal homogeneous solid support comprised of two or more independently selected universal linker subsets, wherein said each of said universal linker subsets is comprised of anchor groups that are protected with protective groups that are orthogonal to each other.
A method for preparing a composite solid support, said method comprising the step of mixing two or more solid support components to provide the composite solid support.
A composite solid support comprised of two or more preloaded solid support components, wherein each of said solid support components is comprised of an independently selected linker and an independently selected first nucleosidic moiety, wherein each nucleosidic moiety is comprised of anchor groups that are protected by protective groups that are orthogonal to each other.
A composite solid support comprising two or more universal solid support components, wherein each of said solid support components is comprised of an independently selected universal linker, wherein each of said independently selected universal linkers is comprised of anchor groups that are protected with protective groups that are orthogonal to each other.
Dependencies are inferred from the claim text.