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US 2006/0149040 A1Published application
Collagen and method for producing same
OwnerJohn Snowdeninventor
Life of this patent
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Abstract
The present invention relates to a method for producing collagen from an animal skin. In particular, the present invention a method for producing collagen from an animal skin comprising the step of applying to said skin an organic acid buffer for sufficient time to enable the extraction of collagen.
Claims43
A method for producing collagen from a mammalian animal skin comprising
- (a) applying to said skin an organic acid buffer and incubating said skin for sufficient time to loosen attached wool or hair;
- (b) mincing or chopping said skin to produce skin pieces;
- and
- (c) extracting said collagen from said skin pieces.
1 more element
A method for producing purified native collagen comprising the steps of:
- a). providing an animal skin;
- b). contacting said skin with organic acid buffer for sufficient time to substantially digest the glycosaminoglycans present in the skin to produce a pelt;
- c). mincing said pelt and exposing to one or more protease(s) to produce a preparation comprising soluble and dispersed collagen;
- d). centrifuging said preparation to isolate soluble native collagen from dispersed collagen;
- and
- e). precipitating said soluble native collagen with salt to produce purified collagen.
3 more elements
A method for producing purified biocompatible collagen comprising the steps of:
- a). providing an animal skin;
- b). contacting said skin with organic acid buffer for sufficient time to substantially digest the glycosaminoglycans present in the skin to produce a pelt;
- c). mincing said pelt and exposing to one or more protease(s) to produce a preparation comprising soluble and dispersed collagen;
- d). centrifuging said preparation to isolate soluble native collagen from dispersed collagen;
- and
- e). precipitating said soluble native collagen with salt to produce biocompatible collagen.
3 more elements
Dependencies are inferred from the claim text.