Method of evaluating compound efficacious in treating obesity
AssigneeBanyu Pharmaceutical Co., Ltd.
InventorsHidehito Kotani, Hiraku Itadani, Hiromits Araki, Kazuhiko Takahashi, Nao Odagiri, Tsutomu Kobayashi
Life of this patent
Estimating the term…
Abstract
Examination of obesity or emaciation is performed based on expression levels of LCE gene or protein in a test tissue or a test cell or a polymorphism of the gene. Evaluation of compounds including screening of therapeutic agents for obesity or emaciation is performed utilizing the nature of LCE gene or protein.
Claims17
A method of evaluating compounds which are effective for treatment or prevention of obesity comprising:
- a)
- i) a step in which a test compound is administered to or contacted with a test animal or a test cell, and
- ii) a step in which it is confirmed whether or not said test compound regulates expression levels of LCE gene or a gene which is functionally equivalent to said gene, in said test animal or test cell or
- b)
- i) a step in which a test compound is contacted with a test animal or a test cell possessing a fusion gene comprising an expression regulatory region of LCE gene and a reporter gene, and
- ii) a step in which expression of said reporter gene in said test animal or test cell is assayed;
- or
- c)
- i) a step in which a test compound is contacted with LCE protein and
- ii) a step in which it is confirmed whether or not said test compound exhibits an effect on the activity of said protein;
- or
- d)
- i) a step in which a test compound is contacted with a plurality of elongase proteins including LCE,
- ii) a step in which the activities of said plurality of elongase proteins are assayed, and
- iii) a step in which test compounds are selected which inhibit LCE activity among said plurality of elongase proteins.
2-4. (canceled)
A method of inhibiting fat synthesis by inhibiting LCE fatty acid synthesis activity.
A method of treating or preventing obesity comprising a step of inhibiting LCE fatty acid synthesis activity.
A method of examining obesity by:
- a) assaying an expression level and a change in expression level of LCE gene in a test tissue or a test cell or
- b) assaying an expression level and a change in expression level of LCE protein in a test tissue or a test cell;
- or
- c) detecting a polymorphism in LCE gene in a test tissue or a test cell;
- or
- d) detecting expression or activity of a protein which affects expression of LCE gene thorough interaction with LCE protein.
15-17. (canceled)
siRNA consisting of the nucleic acids of SEQ ID NOs:
- 23 and 24.
Dependencies are inferred from the claim text.