Counterion exchange process for peptides
OwnerAvi Tovi, Chaim Eidelman +6inventors
InventorsAvi Tovi, Chaim Eidelman, Shimon Shushan, Shai Elster, Hagi Alon, Alexander Ivchenko, Gabriel-Marcus Butilca, Gil Zaovi
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Abstract
The invention encompasses a process for purifying a peptide comprising loading a peptide onto a RP-HPLC column; washing the column with an aqueous solution of a pharmaceutically acceptable counterion salt; and eluting the peptide from the column with a solvent mixture of a organic solvent and an acid of the pharmaceutically acceptable counterion, wherein the aqueous solution has a pH of at least about 6.
Claims17
A process for purifying a peptide comprising:
- a) loading a peptide onto a RP-HPLC column;
- b) washing the column with an aqueous solution of a pharmaceutically acceptable counterion salt;
- and
- c) eluting the peptide from the column with a solvent mixture of an organic solvent and an acid of the pharmaceutically acceptable counterion,
- wherein the aqueous solution has a pH of at least about 6.
Nesiritide citrate having no more than about 0.25% by weight of residual counterion.
Eptifibatide acetate having no more than about 0.25% by weight of residual counterion.
Dependencies are inferred from the claim text.