Glyphosate resistant class i 5-enolpyruvylshikimate-3-phosphate synthase(epsps)
AssigneeMonsanto Technology LLC
InventorsMurtaza Alibhai, Claire Cajacob, Paul Feng, Gregory Heck, Youlin Qi, Stanislaw Flasinski, William Stallings
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Abstract
The compositions and methods disclosed herein provide novel DNA molecules that encode glyphosate resistant EPSPS proteins and plants containing these new proteins. The plants that express the new EPSPS proteins are themselves tolerant to the herbicidal effects of glyphosate.
Claims17
An isolated DNA molecule that encodes a glyphosate resistant class I EPSPS protein, wherein said glyphosate resistant class I EPSPS protein comprises a polypeptide sequence GX4X1X2RX3, where X1 and X2 are any amino acid, X4 is isoleucine or leucine, and X3 is selected from the group consisting of threonine, glycine, cysteine, alanine, and isoleucine.
A DNA construct comprising a promoter that functions in plant cells operably linked to a DNA molecule that encodes a glyphosate resistant class I EPSPS protein, wherein said glyphosate resistant class I EPSPS protein comprises a polypeptide sequence GX4X1X2RX3, where X1 and X2 are any amino acid, and X4 is isoleucine or leucine and X3 is selected from the group consisting of threonine, glycine, cysteine, alanine, and isoleucine.
A method of controlling weeds in a field of glyphosate tolerant crop plants comprising applying to said field of glyphosate tolerant crop plant an effective dose of a glyphosate containing herbicide, wherein said glyphosate tolerant crop plant contains a DNA construct comprising a promoter that functions in plant cells operably linked to a DNA molecule that encodes a chloroplast transit peptide linked to a glyphosate resistant class I EPSPS protein, wherein said glyphosate resistant class I EPSPS protein comprises a polypeptide sequence GIX1X2RX3, where X1 and X2 are any amino acid, and X3 is selected from the group consisting of threonine, glycine, cysteine, alanine, and isoleucine.
A purified EPSPS enzyme comprising a polypeptide sequence GX4X1X2RX3, where X1 and X2 are any amino acid, X4 is isoleucine or leucine, and X3 is selected from the group consisting of threonine, glycine, cysteine, alanine, and isoleucine.
Dependencies are inferred from the claim text.