Computer software products for nucleic acid hybridization analysis
AssigneeAffymetrix, Inc.
InventorsDavid Balaban, Earl Hubbell
Life of this patent
Estimating the term…
Abstract
Methods and computer software products are provided for analyzing gene expression data In one embodiment, multiple probes are used to detect a single transcript The hybridization intensities of each probe is adjusted by dividing the intensities by the affinities of the probes The minimal adjusted hybridization intensity may be used as the measurement of the gene expression
Claims23
A computer implemented method for determining Hybridization between a plurality of nucleic acid probes and a nucleic acid target comprising
- inputting a plurality of hybridization intensities, each of said intensities reflects said hybridization between one of said plurality of said probes and said nucleic acid target,
- adjusting said hybridization intensities for hybridization affinities of said probes to obtain a plurality of adjusted hybridization intensities,
- finding the minimal adjusted Hybridization intensity among said adjusted hybridization intensities;
- and
- indicating said minimal adjusted hybridization intensity as a measurement of said hybridization
A computer software product for determining hybridization between a plurality of nucleic acid probes and a nucleic acid target comprising
- computer program code for inputting a plurality of Hybridization intensities, each of said hybridization intensities reflects said hybridization between one of said plurality of said probes and said nucleic acid target,
- computer program code for adjusting said hybridization intensities for hybridization affinities of said probes to obtain a plurality of adjusted hybridization intensities,
- computer program code for finding the minimal adjusted hybridization intensity among said adjusted hybridization intensities and
- computer program code for indicating said minimal adjusted hybridization intensity as a measurement of said hybridization, and
- a computer readable media for storing said code
A computer-readable medium having computer-executable instructions for performing a method comprising,
- inputting a plurality of hybridization intensities, each of said intensities reflects said hybridization between one of said plurality of said probes and said nucleic acid target,
- adjusting said hybridization intensities for hybridization affinities of said probes to obtain a plurality of adjusted hybridization intensities,
- finding the minimal adjusted hybridization intensity among said adjusted hybridization intensities, and
- indicating said minimal adjusted hybridization intensity as a measurement of said hybridization
A system for comparing nucleic acid probes, comprising
- a processor, and a memory being coupled to the processor, the memory storing a plurality machine instructions that cause the processor to perform a plurality of logical steps when implemented by the processor, said logical steps including
- inputting a plurality of Hybridization intensities, each of said intensities reflects said hybridization between one of said plurality of said probes and said nucleic acid target,
- adjusting said hybridization intensities for hybridization affinities of said probes to obtain a plurality of adjusted hybridization intensities,
- finding the minimal adjusted hybridization intensity among said adjusted hybridization intensities, and
- indicating said minimal adjusted hybridization intensity as a measurement of said hybridization
Dependencies are inferred from the claim text.