Programmable apparatus and method for optimizing and real time monitoring of gene transfection based on user configured arbitrary waveform pulsing train

An apparatus for generating arbitrary waveform pulsing trains for an electroporation process, said apparatus comprising of:

• a customized power module including:
• a direct current (DC) power supply array for providing an output voltage;
• a capacitor;
• an electronic switch coupled to the DC power supply array, wherein the electronic switch provides means for controlling the output voltage from the DC power supply array to the capacitor;
• a pulse width modulation (PWM) controller coupled to the electronic switch, wherein the PWM controller receives an arbitrary waveform pulsing train and command signals for controlling the electronic switch;
• a low pass filter (LPF) coupled to the capacitor, wherein the LPF receives an output waveform pulsing train from the capacitor and removes high frequency spikes in the output waveform pulsing train from the capacitor, producing a filtered waveform pulsing train;
• a polarity switch unit coupled to the low pass filter, wherein the polarity switch unit reverses the polarity of the required pulses in the filtered waveform pulsing train to produce an amplified waveform pulsing train;
• a waveform generator unit coupled to the customized power module, wherein the waveform generator unit provides the arbitrary waveform pulsing train to the customized power module;
• and
• wherein the amplified waveform pulsing train produced by the polarity switch unit substantially corresponds to the arbitrary waveform pulsing train.