Vaccine
OwnerPeter Ertlinventor
Life of this patent
Estimating the term…
Abstract
The invention relates to polynucleotides for DNA vaccination which polynucleotides encode an HIV envelope protein or fragment or immunogenic derivative fused to an additional HIV protein selected from a non-structural protein or capsid protein or fragment or immunogenic derivative thereof. Preferably the HIV envelope molecule is gp120 and preferred fusions include one or more of HIV Nef, Gag, RT or Tat. Preferably the HIV envelope molecule is non-glycosylated in mammalian cells.
Claims34
A polynucleotide that comprises a sequence encoding an HIV envelope protein, fused to at least one sequence encoding an HIV non-structural or capsid protein, operably linked to a heterologous promoter.
A polynucleotide sequence selected from the group of:
- gp120 codon optimized, minus secretion signal—tr Nef,
- gp120 codon optimized, minus secretion signal—tr Nef-mTat,
- gp120 codon optimized, minus secretion signal—Nef-mTat.
- gp120 codon optimized, minus secretion signal—p17/24 Gag-tr Nef,
- gp120 codon optimized, minus secretion signal—p17/24 Gag-tr Nef-mTat,
- gp120 codon optimized, minus secretion signal—p17/24 gag-Nef-mTat,
- gp120 codon optimized, minus secretion signal—p17/24 gag-mNef-mTat,
- gp120 codon optimized, minus secretion signal—p17/24 gag-L1Nef-mTat,
- gp120 codon optimized, minus secretion signal—p17/24 gag-L2Nef-mTat,
- gp120 codon optimized, minus secretion signal—p17/24 gag-LLNef-mTat,
- gp120 codon optimized, minus secretion signal—p17/24 gag-mLLNef-mTat,
- gp120 codon optimized, minus secretion signal—p17/24 gag-mL1Nef-mTat,
- gp120 codon optimized, minus secretion signal—p17/24 gag-mL2Nef-mTat,
- gp120 codon optimized—trNef.
- gp120 codon optimized—trNef-mTat,
- gp120 codon optimized—Nef-mTat,
- Nef-mTat—gp120 codon optimized,
- trNef-mTat—gp120 codon optimized.
- gp120 codon optimized—p17/24 Gag-trNef,
- gp120 codon optimized—p17/24 Gag-trNef-mTat,
- gp120 codon optimized, minus secretion signal—mRT-trNef-p17/24 Gag, and
- mRT-trNef-p17/24 Gag—gp120 codon optimized, minus secretion signal, wherein RT and Gag are codon optimized.
A fusion protein comprising an HIV envelope protein at least one additional HIV protein selected from non-structural or capsid proteins.
29-30. (canceled)
A polynucleotide that comprises a sequence encoding an HIV Tat protein in a fusion with at least two HIV antigens.
(canceled)
Dependencies are inferred from the claim text.