Novel alkaline protease
OwnerRajni Hatti-Kaul, Bo Mattiasson +1inventors
InventorsRajni Hatti-Kaul, Bo Mattiasson, Amare Gessesse
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Abstract
The present invention relates to a novel isolated and purified alkaline protease from Nesterenkonia sp. nov. strain, having the following characteristics: molecular weight of 23 kilodalton, melting temperature of about 74° C. at pH 7-10, calcium independent for activity and stability, maximal protease activity at pH 10, and being a serine protease, as well as a composition comprising the enzyme, method for producing the enzyme, method of degrading proteinaceous material, an isolated strain Nesterenkonia sp. nov..
Claims34
An isolated alkaline protease derived from Nesterenkonia sp. nov. strain deposited at Deutsche Sammlung von Mikroorganismen und Zellkulturen under DSM accession number 15380 on Dec. 20, 2002, having the following characteristics:
- molecular weight of 23 kilo Dalton,
- melting temperature of about 74° C. at pH 7-10,
- calcium independent for activity and stability,
- maximal protease activity at pH 10 and retaining 90% of its original activity between pH 6 and 11, and having 60% of its peak activity at pH 12 and being a serine protease, and retains full activity in the presence of 1% (w/v) SDS and 1% (w/v) Triton X-100 at 25° C.
An isolated Nesterenkonia sp. nov. deposited at Deutsche Sammlung von Mikroorganismen und Zellkulturen under DSM accession number 15380 on Dec. 20, 2002.
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Dependencies are inferred from the claim text.