Vectors for stable gene expression
AssigneeStratatech Corporation
InventorAllen Comer
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Abstract
The present invention relates to expression vectors capable of promoting transgene expression. The expression vectors include site specific recombination elements, insulator elements, and recombinase elements. In particular, the present invention provides methods for obtaining specific and stable integration of nucleic acids into eukaryotic cells through site specific recombination.
Claims27
An expression vector comprising one or more promoters operably linked to one or more genes of interest and a site-specific recombination element flanked by at least one insulator element on both the 5′ and 3′ sides of said recombination element, wherein upon recombination with a chromosome said expression vector produces an insert with the following 5′ to 3′ arrangement:
- site-specific recombination remnant, insulator element, genes of interest operably linked to promoters, insulator element, and site-specific recombination recombination remnant.
A method of expressing a gene of interest in a host cell, comprising:
- a) providing:
- i) a first expression vector comprising a promoter operably linked to said gene of interest and a site-specific recombination element flanked by at least one insulator element on both the 5′ and 3′ sides of said recombination element;
- ii) a second expression vector comprising a promoter operably linked to a recombinase;
- and
- iii) host cells;
- b) introducing said first and second expression vectors into said host cells under conditions such that recombinase protein produced from said second expression vector causes integration of said first expression vector via said recombination element.
Dependencies are inferred from the claim text.