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US 2006/0141625 A1Published application
Expression cassette and vector for transient or stable expression of exogenous molecules
AssigneeBiogen Idec MA Inc.
InventorsWilliam Sisk, Holly Prentice
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Abstract
The disclosure provides an expression cassette and a vector comprising the cassette for expression of a polynucleotide. The expression cassette includes a promoter/enhancer, an intervening region, and a polyadenylation signal domain. Expression systems and methods of using the expression cassette and vector are also provided.
Claims13
An expression cassette comprising
- a human CMV immediate early 1 (HCMV IE1) promoter/enhancer region;
- a polynucleotide of interest;
- and
- a variant human growth hormone (hGHv) polyA signal domain or variant thereof, wherein the hGHv polyA signal is at least 100 nucleotides in length and contains the sequence AATAAA, and
- the polyA variant is at least 92% identical to a hGH polyA signal domain.
2 more elements
Dependencies are inferred from the claim text.