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AssigneeKarsten Koenig
InventorsKarsten Koenig, Uday Tirlapur
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The invention relates to an optical method for targeted transfer of molecules, preferably of DNA, RNA, peptides, amino acids and proteins, into vital cells by means of laser radiation and to an arrangement for implementing the method. The object of the invention, to find a novel possibility for targeted molecule transfer into the interior of vital cells, particularly the transfer of DNA, RNA, peptides, amino acids and proteins, which achieves a high transfer efficiency while extensively excluding destructive side effects such as a lethal effect on a treated cell, is met according to the invention in that cellular membranes are opened transiently for the molecule transfer by multiple laser pulses in the microjoule range or less and a pulsed, near-infrared laser beam with a pulse width in the femtosecond range is directed in each instance to a submicrometer spot of a membrane of the vital cell for an irradiation period of less than one second.
1-17. (canceled)
A method for transferring molecules into vital cells by laser radiation, preferably for targeted transfer of DNA, RNA, peptides, amino acids and proteins into vital cells and for the transfection of plant cells, animal cells and human cells, particularly for the production of drugs such as synthetic vaccines, comprising the steps of:
An arrangement for targeted transient molecule transfer into vital cells by means of a laser beam, particularly for the transfer of DNA, RNA, peptides, amino acids or proteins, in which the molecules are moved from an extracellular environment through an increased permeability of a membrane into the interior of a cell, with a laser whose laser beam is focused on the cell with a short pulse duration, comprising:
Dependencies are inferred from the claim text.