Device and method for parallel, automated cultivation of cells in technical conditions
AssigneeTechnische University Munchen
InventorsRobert Puskeiler, Dirk Weuster-Botz, Karl-Heinz Zacher
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Abstract
The invention relates to a device for the cultivation of cells in liquid columns measured on a millimetre scale. Said device comprises a container for receiving a liquid culture suspension of the cells, a mixing element for mixing the culture suspension in the container around a mixing axis. The container and/or the mixing element are configured in such a manner that the speed of the flow is modified either locally and/or temporally along a streamline or flow line around the mixing axis.
Claims54
Device for cultivating cells in liquid columns on a millilitre scale, having a container for receiving a liquid culture suspension of the cells, an agitation system for mixing the culture suspension in the container about a mixing axis, characterised in that at least one of the container and the agitation system are configured in such a manner that the flow velocity is modified at least one of locally and temporally along a streamline or flow line about the agitation axis.
Agitation system having a basic body which, in operation, has an upper side and an underside, characterised in that, in the basic body, at least one first through-channel is disposed, the first opening of which is situated at least partially on the underside of the basic body and the second opening of which is situated on at least one of the upper side and laterally on the basic body.
Method for cultivating cells in liquid columns on a millilitre scale, at least one cell suspension being moved in a container in such a manner that the cell suspension forms an annular flow, characterised in that the flow velocity of the annular flow is modified at least one of locally and temporally along a streamline.
Dependencies are inferred from the claim text.