Capillary perfused bioreactors with multiple chambers

A bioreactor comprising:

• i. a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving cells and a liquid medium;
• ii. an inlet port formed in the first substrate;
• iii. a first connection channel formed in the first substrate, wherein the first connection channel is in fluid communication with the inlet port and the chamber for allowing a stream of substance to be delivered to the chamber;
• iv. an outlet port formed in the first substrate;
• v. a second connection channel formed in the first substrate, wherein the second connection channel is in fluid communication with the outlet port and the chamber for allowing a stream of substance to be removed from the chamber;
• and
• vi. confining means positioned in a region in the chamber proximate to the first connection channel to confine the cells.

A bioreactor comprising:

• i. a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving cells and a liquid medium;
• ii. an inlet port formed in the first substrate;
• iii. a first connection channel formed in the first substrate, wherein the first connection channel is in fluid communication with the inlet port and the chamber for allowing a stream of substance to be delivered to the chamber;
• iv. an outlet port formed in the first substrate;
• v. a second connection channel formed in the first substrate, wherein the second connection channel is in fluid communication with the outlet port and the chamber for allowing a stream of substance to be removed from the chamber, and
• vi. confining means positioned in the chamber to form a confinement region to confine the cells therein.