Biotechnological process for neutralizing alkaline beverage industrial waste water
AssigneeCouncil of Scientific & Industrial Reseach
InventorsRita Kumar, Anil Kumar
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Abstract
The present invention provides a process of neutralizing beverage industrial wastewater by a bacterial strain Exiguobacterium sp. isolated in India, which strain is capable of bring down the pH of wastewater from 12.00 to 7.00 units within 1 to 1.5 hours.
Claims21
A bacterial isolate Exiguobacterium sp. (MTCC 5183) deposited at International Depository at IMTECH, Sector 39A, Chandigarh, India recognized by Budapest Treaty.
A process for preparing a bacterium isolate of Exiguobacterium sp. (MTCC 5183) useful in neutralization of alkaline waste water of beverage industry having a pH in the range of 12.00-11.5, deposited at International Depository at IMECH, Sector 39A, Chandigarh, India recognized by Budapest Treaty, the process comprising the steps of:
- (a) enriching activated sludge contaminated with bacteria by providing a sludge extract in alkaline baccilus medium;
- (b) culturing the bacteria;
- (c) isolating the bacteria by centrifuging the culture obtained from step (b) after attaining the desired growth to obtain a pellet of bacterial cells;
- (d) dissolving the pellet obtained from step (c) in phosphate buffer;
- (e) neutralizing alkaline wastewater of beverage industry having pH in the range of 12.00-11.5 by adding the bacterial pellet obtained from step (d) therein.
Dependencies are inferred from the claim text.