Methods and compositions related to argonaute proteins
OwnerLeemor Joshua-Tor, Ji-Joon Song +4inventors
InventorsLeemor Joshua-Tor, Ji-Joon Song, Gregory Hannon, Jidong Liu, Michelle Carmell, Fabiola Rivas
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Abstract
This invention provides methods and compositions related to Argonaute proteins and, in certain embodiments, the applications of these methods and compositions to treatment and therapeutics based on RNAi.
Claims23
A crystalline Argonaute.
2-5. (canceled)
A data array comprising the atomic coordinates of an Argonaute protein as set forth in Table 3.
An electronic representation of a crystal structure of an Argonaute protein or a portion thereof.
12-13. (canceled)
A method of identifying an agent that modulates the activity of an RNAi construct comprising:
- (a) providing an isolated or recombinant Argonaute protein;
- and
- (b) assaying the expression and/or activity of said Argonaute protein in the presence of a candidate agent,
- wherein a change in the expression and/or activity of said Argonaute protein in the presence of a candidate agent is indicative of said candidate agent capable of modulating the activity of an RNAi construct.
A composition for targeted gene inhibition comprising an agent that modulates the RNase activity of an Argonaute protein.
A cell line that overexpresses an Argonaute protein.
A nucleic acid composition comprising:
- (a) a first nucleic acid comprising an RNAi construct and
- (b) a second nucleic acid encoding an Argonaute protein.
Dependencies are inferred from the claim text.