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US 2006/0141599 A1Published application
Crystals of Glucokinase and methods of growing them
AssigneeHoffmann-La Roche Inc.
InventorsWendy Corbett, Robert Crowther, Pete Dunten, R. Ursula Kammlott, Christine Lukacs
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Abstract
Crystalline forms of mammalian Gluckokinase of sufficient size and quality to obtain structural data by X-ray crystallography are presented. Methods of growing such crystals are also disclosed.
Claims4
A co-crystal of mammalian Glucokinase and a ligand bound to an allosteric site of the Glucokinase, wherein
- the co-crystal has unit cell dimensions of:
- a and b are from about 79 Å to about 80.2 Å;
- c is from about 318 Å to about 325 Å;
- α and β are 90°;
- and
- γ is 120°;
- and the co-crystal has P6(5)22 symmetry.
4 more elements
A crystal of mammalian Glucokinase, wherein
- the crystal has unit cell dimensions of:
- a and b are from about 79 Å to about 80.2 Å;
- c is from about 318 Å to about 325 Å;
- α and β are 90°;
- and
- γ is 120°;
- and the crystal has P6(5)22 symmetry.
4 more elements
A process for co-crystalizing mammalian Glucokinase and an allosteric ligand of Glucokinase, the process comprising:
- providing a buffered, aqueous solution of about 9 to about 22 mg/ml the mammalian Glucokinase;
- adding a molar excess of the allosteric ligand to the aqueous solution of mammalian Glucokinase;
- and
- growing crystals by vapor diffusion using a buffered reservoir solution of about 16% to about 25% PEG, about 0% w/v to about 30% w/v glucose and about 8 to about 10 mM DTT, wherein the PEG has an average molecular weight of about 8,000 to about 10,000.
1 more element
Dependencies are inferred from the claim text.