Simultaneous anaerobic production of isoamyl acetate and succinic acid
AssigneeRice University
InventorsKa-Yiu San, Ailen Sanchez, George Bennett, Cheryl Dittrich
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Abstract
In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method is exemplified with null mutations in ldhA, adhE, ackA-pta and overexpression of pyruvate carboxylase and alcohol acetyltransferase. This strain produces higher levels of both isoamyl acetate and succinate.
Claims20
A metabolically engineered bacterial cell wherein said bacterial cell comprises:
- a) decreased activity of lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH);
- and
- b) an exogenous alcohol acetyltransferase (ATF).
A method of producing increased levels of isoamyl acetate and succinate comprising:
- a) culturing a bacterial cell wherein said bacterial cell comprises:
- i) decreased activity of lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH), and
- ii) an exogenous alcohol acetyltransferase (ATF);
- and
- b) isolating isoamyl acetate.
A metabolically engineered bacterial cell, wherein said cell comprises:
- a) a disruption of of lactate dehydrogenase (ldhA) and alcohol dehydrogenase (adhE);
- and
- b) an expression vector encoding alcohol acetyltransferase (atf).
Dependencies are inferred from the claim text.