Dna encoding favin-adenine-dinucleotide-dependent-d-erythronate-4-phosphate-de-hydrogenase, pdxr, and microbial production of vitamin b6

A process for the biological production of vitamin B6 which comprises cultivating a host cell transformed or transfected by an isolated DNA or by a vector or plasmid comprising the isolated DNA under the condition conductive to the production of vitamin B6, and recovering vitamin B6 from the culture, wherein the isolated DNA comprises a nucleotide sequence that encodes PdxR, which is a flavin adenine dinucleotide-dependent D-erythronate 4-phosphate dehydrogenase, selected from the group consisting of:

• (a) a DNA sequence identified by SEQ ID NO:
• 1 or the complementary strand thereof;
• (b) a DNA sequence which hybridizes under standard conditions to the DNA sequence complementary to the DNA sequence defined in (a) or a fragment thereof, and encodes a polypeptide having the activity of flavin adenine dinucleotide-dependent D-erythronate 4-phosphate dehydrogenase;
• (c) a DNA sequence which codes for a polypeptide having the amino acid sequence encoded by the DNA sequence of (a) or (b);
• (d) a DNA sequence which is identical to the extent of at least 80% to a DNA which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:
• 2, and encodes a polypeptide having the activity of flavin adenine dinucleotide-dependent D-erythronate 4-phosphate dehydrogenase;
• and
• (e) a DNA sequence which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 80% to the amino acid sequence of SEQ ID NO:
• 2, and encodes a polypeptide having the activity of flavin adenine dinucleotide-dependent D-erythronate 4-phosphate dehydrogenase.