L-glutamic acid-producing microorganism and a method for producing l-glutamic acid
AssigneeAjinomoto Co., Inc.
InventorsJun Nakamura, Seiko Hirano, Hisao Ito
Life of this patent
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Abstract
A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells.
Claims29
A coryneform bacterium having L-glutamic acid-producing ability, wherein said coryneform bacterium is modified so that L-glutamic acid-producing ability of the strain is enhanced as compared to a non-modified strain, wherein said bacterium is modified in a manner selected from the group consisting of:
- a) enhancing the expression of a yggB gene or enhancing the activity of the yggB protein,
- b) introducing a mutant-type yggB gene,
- c) enhancing the expression of a mutant-type yggB gene, and
- d) combinations thereof.
A mutant-type yggB gene selected from the group consisting of:
- (a) a gene encoding the amino acid sequence of SEQ ID NO:
- 8,
- (b) a gene encoding a protein having a homology of not less than 80% to SEQ ID NO:
- 8, and having a function to increase L-glutamic acid-producing ability of coryneform bacterium in the presence of excess biotin when said gene is introduced into the coryneform bacterium,
- (c) a gene encoding amino acid sequence of SEQ ID NO:
- 20,
- (d) a gene encoding a protein having a homology of not less than 80% to SEQ ID NO:
- 20, and having a function to increase L-glutamic acid-producing ability of coryneform bacterium in the presence of excess biotin when said gene is introduced into the coryneform bacterium,
- (e) a gene encoding amino acid sequence of SEQ ID NO:
- 22,
- (f) a gene encoding a protein having a homology of not less than 80% to SEQ ID NO:
- 22, and having a function to increase L-glutamic acid-producing ability of coryneform bacterium in the presence of excess biotin when said gene is introduced into the coryneform bacterium,
- (g) a gene encoding amino acid sequence of SEQ ID NO:
- 24,
- (h) a gene encoding a protein having a homology of not less than 80% to SEQ ID NO:
- 24, and having a function to increase L-glutamic acid-producing ability of coryneform bacterium in the presence of excess biotin when said gene is introduced into the coryneform bacterium,
- (i) a gene encoding amino acid sequence of SEQ ID NO:
- 64,
- (j) a gene encoding a protein having a homology of not less than 80% to SEQ ID NO:
- 64, and having a function to increase L-glutamic acid-producing ability of coryneform bacterium in the presence of excess biotin when said gene is introduced into the coryneform bacterium,
- (k) a gene encoding amino acid sequence of SEQ ID NO:
- 70,
- (1) a gene encoding a protein having a homology of not less than 80% to SEQ ID NO:
- 70, and having a function to increase L-glutamic acid-producing ability of coryneform bacterium in the presence of excess biotin when it is introduced into the coryneform bacterium.
- (m) a gene encoding amino acid sequence of SEQ ID NO:
- 74,
- (n) a gene encoding a protein having a homology of not less than 80% to SEQ ID NO:
- 74, and having a function to increase L-glutamic acid-producing ability of coryneform bacterium in the presence of excess biotin when said gene is introduced into the coryneform bacterium.
A method for producing a coryneform bacterium having a mutant-type yggB gene comprising:
- a) inoculating a coryneform bacterium which is deficient in a gene encoding α-ketoglutarate dehydrogenase into a medium containing excess biotin, and
- b) selecting a strain that is capable of causing accumulation of L-glutamic acid in the medium.
A method for producing a coryneform bacterium having a mutant-type yggB gene comprising:
- a) inoculating a coryneform bacterium having a yggB gene which contains a randomly introduced in vitro mutation into a medium containing excess biotin, and
- b) selecting a strain that is capable of causing accumulation of L-glutamic acid in the medium.
A method for producing a coryneform bacterium having a mutant-type yggB gene comprising:
- a) inoculating a coryneform bacterium having a transposable element randomly introduced onto a chromosome into a medium containing excess biotin, and
- b) selecting a strain that is capable of causing accumulation of L-glutamic acid in the medium.
A method for producing a coryneform bacterium having a mutant-type yggB gene comprising:
- a) inoculating a coryneform bacterium into a medium containing L-glutamic acid analogs, and
- b) selecting a strain which grows on the medium containing L-glutamic acid analogs.
Dependencies are inferred from the claim text.