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AssigneeBASF SE
InventorsR Yocum, Thomas Patterson, Janice Pero, Theron Hermann
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The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.
A process for the enhanced production of pantothenate, comprising culturing a microorganism having a deregulated methylenetetrahydrofolate (MTF) biosynthetic pathway, under conditions such that pantothenate production is enhanced.
A process for the enhanced production of pantothenate, comprising culturing a microorganism having
A process for the enhanced production pantothenate, comprising culturing a microorganism having a deregualted pantothenate biosynthetic pathway, a deregulated isoleucine-valine (ilv) biosynthetic pathway, and a deregulated methylenetetrahydrofolate (MTF) biosynthetic pathway deregulated, such that production of pantothenate is enhanced.
A process for the production pantothenate, comprising culturing a microorganism having a deregulated pantothenate biosynthetic pathway, a deregulated isoleucine-valine (ilv) biosynthetic pathway, and a deregulated methylenetetrahydrofolate (MTF) biosynthetic pathway, such that at least 50 g/L pantothenate is produced after 36 hours of culturing the microorganism.
A process for the production pantothenate, comprising culturing a microorganism having a deregulated pantothenate biosynthetic pathway, a deregulated isoleucine-valine (ilv) biosynthetic pathway, and a deregulated methylenetetrahydrofolate (MTF) biosynthetic pathway deregulated, such that at least 60 g/L pantothenate is produced after 48 hours of culturing the microorganism.
The process of any one of the preceding claims, wherein pantothenate production is further enhanced by regulating pantothenate kinase activity.
The process of any one of the above claims, wherein said microorganism is cultured under conditions of excess serine.
A process for producing pantothenate comprising culturing a microorganism having a deregulated pantothenate biosynthetic pathway under conditions of excess serine, such that pantothenate in produced.
The process of any one of the above claims, wherein said microorganism has the pantothenate biosynthetic pathway deregulated such that pantothenate production is independent of β-alanine feed.
The process of any one of the above claims wherein the microorganism is a Gram positive microorganism.
The process of any one of the above claims wherein the microorganism belongs to the genus Bacillus.
The process of any one of the above claims, wherein the microorganism is Bacillus subtilis.
A product synthesized according to the process of any one of the above claims.
A composition comprising pantothenate produced according to the process of any one of the above claims.
A recombinant microorganism for the enhanced production of pantothenate, said microorganism having a deregulated pantothenate biosynthetic pathway, and a deregulated methylenetetrahydrofolate (MTF) biosynthetic pathway.
A recombinant microorganism for the enhanced production of pantothenate, said microorganism having a deregulated pantothenate biosynthetic pathway, a deregulated methylenetetrahydrofolate (MTF) biosynthetic pathway, and a deregulated isoleucine-valine (ilv) pathway.
A process for producing pantothenate comprising culturing a recombinant microorganism having:
A process for producing pantothenate comprising culturing a recombinant microorganism having:
A process for producing pantothenate comprising culturing a recombinant microorganism having:
A process for producing pantothenate comprising culturing a recombinant microorganism having:
A process for producing pantothenate comprising culturing a recombinant microorganism having:
A process for producing pantothenate comprising culturing a recombinant microorganism having:
A process for producing pantothenate comprising culturing a recombinant microorganism having:
Dependencies are inferred from the claim text.