Method of protein synthesis
AssigneeKorea Research Instituted of Bioscience and Biotechnology
InventorsJae-Gu Pan, Heung-Chae Jung, Sooan Shin
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Abstract
The present invention relates to a method of protein synthesis and, more particularly, to a method of effective protein synthesis by regulating the expression of a protein in a host cell, wherein said host cell is transformed with an expression vector comprising a promoter as well as a DNA fragment for a gene that encodes a desired protein, wherein said promoter has an inductive activity for transcription during the resting stage of cell growth and also the induction of said protein expression can be controlled by varying culturing conditions.
Claims22
A method of producing a desired protein by gene recombination, the method comprising the steps of:
- (a) constructing a gene expression vector comprising a gene that encodes the desired protein and a promoter which has an inductive activity for transcription during the resting stage of cell growth, wherein the transcription is induced by an organic acid compound;
- (b) introducing said gene expression vector into a host cell;
- (c) inducing the expression of said desired protein by culturing said host cell in culture medium;
- and
- (d) recovering the desired protein.
A vector comprising a gene that encodes a desired protein and a promoter which has an inductive activity for transcription during the resting stage of cell growth, wherein the transcription is induced by an organic acid compound.
A method of producing a desired protein with cell density culture of host cells greater than 40 g/L by gene recombination comprising
- (a) constructing a gene expression vector comprising a gene that encodes the desired protein and a promoter which has an inductive activity for transcription during the resting stage of cell growth, wherein the transcription is induced by an organic acid;
- (b) introducing said gene expression vector into a host cell;
- (c) inducing the expression of said desired protein by culturing said host cell in a culture medium;
- and
- (d) recovering the desired protein.
A vector comprising a promoter containing no CRP (catabolite repression protein) binding site for acs promoter.
A vector comprising a promoter containing tandemly repeated acs promoters or the acs promoter without a CRP binding site.
Dependencies are inferred from the claim text.