Variants of corticotropin releasing hormone receptor type 1 and uses thereof
AssigneeThe University of Tennessee Research Corporation
InventorsAlexander Pisarchik, Andrzej Slominski
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Abstract
The present invention identifies four new isoforms of human corticotropin releasing hormone receptor type 1 (CRH-R1e, 1f, 1g and 1h) and three new isoforms of mouse corticotropin releasing hormone receptor type 1 (mCRH-R1c, 1e and 1f). The data indicate that polymorphism of CRH-R1 expression is related to anatomic location, skin physiological or pathologic status, specific cell type, external stress (UV), and that cAMP dependent pathways and TPA may regulate CRH-R1.
Claims32
DNA encoding a corticotropin releasing hormone receptor type 1 protein comprising an amino acid sequence selected from the group consisting of SEQ ID No. 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 31, 32, 33, 34, 35, 36, and 37.
A method of protecting skin cells against damage induced by an environmental factor, comprising the step of:
- inducing the expression of corticotropin releasing hormone receptor type 1g in said skin cells, wherein the expression of said receptor type 1g protects said skin cells against said damage.
A method of screening for a compound that induces the expression of corticotropin releasing hormone receptor type 1f or 1g, comprising the steps of:
- contacting said compound with skin cells;
- and
- determining the expression of said receptor type 1f or 1g in cells that are or are not treated with said compound, wherein increased expression of said receptor type 1f or 1g in treated cells compared to untreated cells indicates said compound induces expression of said receptor type 1f or 1g.
A method of regulating an extracellular concentration of corticotropin releasing hormone or corticotropin releasing hormone related peptides, comprising the step of:
- administering corticotropin releasing hormone receptor type 1e or 1h to an individual, wherein said receptor type 1e or 1h regulates said extracellular concentration of corticotropin releasing hormone or corticotropin releasing hormone related peptides by binding and slowly releasing said hormone or said peptides in said individual.
Dependencies are inferred from the claim text.