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US 2006/0141574 A1Published application
Method for producing monoclonal antibodies
AssigneeWyeth
InventorsClive Wood, Randal Kaufman
Life of this patent
Estimating the term…
Abstract
An improved method for the production of monoclonal antibodies is disclosed.
Claims21
A method of optimizing the expression level of an antibody or a fragment thereof, which comprises:
- (a) producing a eukaryotic host cell containing and capable of expressing a first DNA sequence encoding at least an antibody heavy chain, said first DNA sequence being associated with a first heterologous selectable amplifiable marker gene, and a second DNA sequence encoding at least an antibody light chain, said second DNA sequence being associated with a second heterologous selectable amplifiable marker gene;
- (b) culturing said host cell in a suitable culture medium;
- and
- (c) differentially amplifying said amounts of said first and second DNA sequences with appropriate selective agents to allow maximized production of said antibody or fragment thereof.
1 more element
2-6. (canceled)
A method of optimizing expression of an antibody or fragment thereof comprising:
- (a) producing a eukaryotic host cell containing and capable of expressing a first DNA sequence encoding at least an antibody heavy chain variable region or an antigen binding portion thereof, said first DNA sequence being associated with a heterologous selectable marker gene, and a second DNA sequence encoding at least an antibody light chain variable region or an antigen binding portion thereof, said second DNA sequence being associated with a heterologous selectable amplifiable marker gene;
- (b) culturing said host cell in a suitable selective culture medium;
- and
- (c) differentially amplifying said second DNA sequence with appropriate selective agents to optimize expression of said antibody or fragment thereof.
1 more element
A method of optimizing expression of an antibody or fragment thereof comprising:
- (a) producing a eukaryotic host cell containing and capable of expressing a first DNA sequence encoding at least an antibody light chain variable region or an antigen binding portion thereof, said first DNA sequence being associated with a heterologous selectable marker gene, and a second DNA sequence encoding at least an antibody heavy chain variable region or an antigen binding portion thereof, said second DNA sequence being associated with a heterologous selectable amplifiable marker gene;
- (b) culturing said host cell in a suitable selective culture medium;
- and
- (c) differentially amplifying said second DNA sequence with appropriate selective agents to optimize expression of said antibody or fragment thereof.
1 more element
Dependencies are inferred from the claim text.