Method for promoting cell growth and increasing the production of the expressed target gene products

A method to increase the amount of the target gene expression, comprising:

• (a) constructing a recombinant vector containing aspartase coding sequence, wherein said recombinant vector including a promoter and one or more aspartase coding sequences manipulated to link and insert into the downstream of the promoter;
• (b) constructing a recombinant vector containing target gene to be expressed;
• (c) co-transforming said recombinant vector in step (a) and in step (b) into host cells and producing recombinant host cells;
• and
• (d) culturing said host cells in culture medium and making said cells to express aspartase and target gene products, wherein said aspartase can increase the amount of the target gene expression.

A method to increase the amount of the target gene expression, comprising:

• (a) constructing a recombinant vector containing aspartase coding sequence and target gene to be expressed;
• (b) transforming said recombinant vector in step (a) into host cells and producing recombinant host cells;
• and
• (c) culturing said host cells in step (b) in culture medium and inducing said cells to express aspartase and target gene products, wherein said aspartase can increase the amount of the target gene expression.

A method to increase the amount of the target gene expression, comprising:

• (a) inserting one or more aspartase coding sequence into chromosomes of host cells to produce recombinant host cells;
• (b) constructing a recombinant vector containing target gene to be expressed;
• (c) transforming said recombinant vector in step (b) into recombinant host cells in step (a) and producing recombinant host cells;
• and
• (d) culturing said recombinant host cells of steps (c) in culture medium and inducing said recombinant host cells to express aspartase and target gene products, wherein said aspartase can increase the amount of the target gene expression.