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US 2006/0141570 A1Published application
Intein-mediated protein purification using in vivo expression of an aggregator protein
OwnerDavid Wood, Mahmoud Banki +1inventors
InventorsDavid Wood, Mahmoud Banki, Tillman Gerngross
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Abstract
Purification of recombinant proteins is performed by expressing in a host cell a fusion protein comprising: (a) a product protein domain, (b) an intein, and (c) at least one aggregator protein domain, wherein the aggregator protein domain comprises a protein that is capable of specific association with granules of polyhydroxyalkanoate (PHA).
Claims27
A fusion protein comprising:
- (a) a product protein domain,
- (b) a self-cleaving intein, and
- (c) at least one aggregator protein domain capable of specific association with granules of polyhydroxyalkanoate (PHA);
- wherein the intein is located between the product protein domain and the aggregator protein domain.
1 more element
Dependencies are inferred from the claim text.