Novel fibroblast growth factor and uses thereof
OwnerMeera Patturajan, Isabelle Millet +6inventors
InventorsMeera Patturajan, Isabelle Millet, Sampath Kumar, Charles Miller, Peter Mezes, Denise Lepley, Catherine Burgess, Richard Shimkets
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Abstract
The present invention generally relates to nucleic acids, proteins, and antibodies. The invention relates more particularly to nucleic acid molecules, proteins, and antibodies of Fibroblast Growth Factor-20 (FGF-22), or its fragments, derivatives, variants, homologs, analogs, or a combination thereof.
Claims25
An isolated nucleic add molecule selected from the group consisting of:
- (a) a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 3, 5, 6, 16, 18, 29, nucleotides 7-453 of SEQ ID NO:9, nucleotides 12-524 of SEQ ID NO:
- 10, nucleotides 17-518 of SEQ ID NO:
- 11, nucleotides 12-530 of SEQ ID NO:
- 12, nucleotides 11-457 of SEQ ID NO:
- 14, nucleotides 4-453 of SEQ ID NO:
- 20, nucleotides 7-456 of SEQ ID NO:
- 22, nucleotides 8-457 of SEQ ID NO:
- 23, nucleotides 8-446 of SEQ ID NO:
- 25, and nucleodies 8-446 of SEQ ID NO:
- 27;
- (b) a nucleic acid molecule encoding a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 2, 4, 7, 13, 15, 17, 19, 21, 24, 26 28, and 30;
- (c) a nucleic acid molecule hybridizes under stringent conditions to a nucleotide sequence of SEQ ID NO:
- 5 or nucleotides 7-456 of SEQ ID NO:
- 22, or a complement of said nucleic acid molecule, and wherein said stringent conditions comprise a salt concentration from about 0.1 M to about 1.0 M sodium ion, a pH from about 7.0 to about 8.3, a temperature is at least about 60° C., and at least one wash in 0.2×SSC, 0.01% BSA;
- (d) a fragment of an nucleic acid molecule of any of (a)-(c);
- and
- (e) a complement of an nucleic acid molecule of any of (a)-(d).
An isolated protein selected from the group consisting of:
- (a) a protein comprising an amino acid sequence of SEQ ID NO:
- 2, 4, 7, 13, 15, 17, 19, 21, 24, 26, 28 or 30;
- (b) a protein with one or more amino acid substitutions to the protein of (a), wherein said substitutions are no more than 15% of the amino acid sequence of SEQ ID NO:
- 2, 4, 7, 13, 15, 17, 19, 21, 24, 26, 28 or 30, and wherein said protein with one or more amino acid substitutions retains cell proliferation stimulatory activity;
- and
- (c) a fragment of the protein of (a) or (b).
An isolated polypeptide comprising an amino acid sequence, wherein said amino acid sequence has one or more conservative amino acid substitutions relative to SEQ ID NO:
- 2, 4, 7, 13, 15, 17, 19, 21, 24, 26, 28 or 30.
An isolated polypeptide comprising an amino acid sequence, wherein said amino acid sequence is a fragment of SEQ ID NO:
- 2, 4, 7, 13, 15, 17, 19, 21, 24, 26, 28 or 30, and wherein said fragment retains cellular proliferation stimulatory activity.
Dependencies are inferred from the claim text.