Process for preparing microbial consortium useful as seeding material for BOD analysis of pulp and paper industrial wastewater
AssigneeCouncil of Scientific and Industrial Research
InventorsRita Kumar, Shikha Rastogi, Anil Kumar
Life of this patent
Estimating the term…
Abstract
The present invention provides a seeding material for accurate and reproducible monitoring of BOD load of pulp and paper wastewater, wherein the bacteria are isolated from various locations in India and comprise Micrococcus sp., Staphylococcus sp., Kurthia zopfii, Alcaligenes faecalis, and Pseudomonas aeruginosa, the microbial composition being capable of exerting an appropriate BOD for pulp and paper industrial wastewater due to the specificity of bacterial stows present therein towards the refractory organic compounds found in these effluents.
Claims44
A synergistic microbial consortium for use as a seeding material for estimation of accurate and reproducible biochemical oxygen demand of pulp and paper industrial waste water, the composition comprising five bacterial strains, Micrococcus sp. (MTCC 6602), Staphylococcus sp. (MTCC 6603), Kurthia zopfii (MTCC 6604), Alcaligenes faecalis (MTCC 6719) and Pseudomonas aeruginosa (MTCC 6605) deposited at International Depository at IMTECH Sector 39A, Chandigarh, India.
A process for preparing a microbial consortium for use as a seeding material for estimation of accurate and reproducible biochemical oxygen demand of pulp and paper industrial waste water, the consortium comprising five bacterial strains, Micrococcus sp. (MTCC 6602), Staphylococcus sp. (MTCC 6603), Kurthia zopfii (MTCC 6604), Alcaligenes faecalis (MTCC 6719) and Pseudomonas aeruginosa (MTCC 6605) deposited at International Depository at IMTECH, Sector 39A, Chandigarh, India, the process comprising inoculating individual bacterial strains of the bacteria separately in a nutrient broth incubating the cultures, measuring cell colony growth at periodic intervals till required growth is attained, mixing the individual cultures in desired proportions to form a cell suspension, centrifuging the cell suspension to obtain a cell pellet and washing the cell pellet, re-centrifuging the cell pellet to obtain a final cell pellet and suspending the final cell pellet in phosphate buffer.
A method for estimating biochemical oxygen demand of pulp and paper industrial wastewater using a consortium comprising five bacterial strains, Micrococcus sp. (MTCC 6602), Staphylococcus sp. (MTCC 6603), Kurthia zopfii (MTCC 6604), Alcaligenes faecalis (MTCC 6719) and Pseudomonas aeruginosa (MTCC 6605) deposited at International Depository at IMTECH, Sector 39A, Chandigarh, India, the process comprising treating pulp and paper industrial wastewater with the microbial consortium and analysing the biochemical oxygen demand thereof.
A method for estimating biochemical oxygen demand of pulp and paper industrial wastewater using a consortium comprising five bacterial strains, Micrococcus sp. (MTCC 6602), Staphylococcus sp. (MTCC 6603), Kurthia zopfii (MTCC 6604), Alcaligenes faecalis (MTCC 6719) and Pseudomonas aeruginosa (MTCC 6605) deposited at International Depository at IMTECH, Sector 39A, Chandigarh, India, the process comprising:
- (a) isolating bacterial strains from soil mu sell pulp and paper industries;
- (b) culturing the strains on a nutrient medium to obtain pure cultures;
- (c) inoculating isolated bacterial strains individually in nutrient medium and incubating them at ambient temperature under gentle shaking for a period of 12-18 hrs;
- (d) observing optical density of the cultures at 620-650 nm till desired growth is attained;
- (e) harvesting grown cells obtained in step (d) by centrifuging for 15-25 min at a temperature in the range of 4 to 10° C. to obtain a cell pellet;
- (f) washing cell pellet obtained in step (e) by dissolving in 10-100 mM phosphate buffer having pH in the range of 6.5-7.5 followed by recentrifugation;
- (g) repeating step (f) for giving a second washing to the obtained cell pellet;
- (h) dissolving pellet obtained from step (g) in desired volume of 10-100 mM phosphate buffer with pH in range of 6.5-7.5 to obtain a seed for BOD analysis of pulp and paper wastewater;
- (i) testing individual pure cultures of isolated bacterial strains obtained in step (h) for analyzing BOD of pulp and paper industrial wastewater;
- (j) verifying BOD values obtained in step (i) using a reference standard;
- (k) comparing the BOD values obtained in step (i) and (j) with values obtained using a synthetic seed;
- (l) selecting bacterial strains which have BOD values equal to or more than BOD values obtained using the synthetic seed as seeding material observed in step (k);
- (m) mixing bacterial strains selected in step (l) in various combinations to obtain different microbial consortia;
- (n) obtaining cell pellet of each microbial consortium following the method of steps c, d, e, f, g and h;
- (o) testing seeds as obtained in step (n) for BOD analysis of pulp and paper wastewater;
- (p) selecting the optimal seed tom results as obtained in step (o).
Dependencies are inferred from the claim text.