Bacterial test method by glycated label binding
OwnerMichael Pugia, Manju Basu +2inventors
InventorsMichael Pugia, Manju Basu, Robert Hatch, James Profitt
Life of this patent
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Abstract
A method for measuring the bacteria content of fluids such as urine and blood, in which a glycoprotein or glycopeptide is attached to the bacteria and a label attached to or inherent to the glycoprotein or glycopeptide provides a means for determining the amount of bacteria present. A preferred glycoprotein is alkaline phosphatase, which is an enzyme capable of attaching to all bacteria present in the fluid sample and inherently includes a label moiety in that color can be developed by addition of known reagents.
Claims21
A method for measuring the bacteria content of fluids comprising:
- a. binding an effective amount of a glycoprotein or glycopeptide with bacteria contained in a sample of fluid, said glycoprotein or glycopeptide having a binding constant to bacteria of at least 106 and at least 100 binding sites, said glycoprotein or glycopeptide consisting of proteins or peptides linked through nitrogen or oxygen bonds to glycosidic groups selected from the group consisting of Gal, GlcNAc, SA, Man, Glc, GalNAc and combinations thereof.
- b. separating excess unbound glycoprotein or glycopeptide from said fluid sample after reacting said glycoprotein or glycopeptide with bacteria in said sample in step (a);
- c. measuring the amount of said glycoprotein or glycopeptide remaining after separating said excess unbound glycoprotein or glycopeptide in step (b) by detecting a label added to said glycoprotein or glycopeptide before the binding with bacteria or adding a label after separating said excess unbound glycoprotein or glycopeptide;
- and
- d. determining the bacteria content of said sample as related to the amount of said label measured in step (c).
A method of measuring the bacteria content of fluids comprising;
- (a) binding an effective amount of a glycoprotein or glycopeptide with bacteria contained in a sample of fluid, said glycoprotein or glycopeptide consisting of at least one member of the group consisting of albumin, prealbumin, bikunin, uristatin, Tamm-Horsfall glycoprotein, alpha-1-Antitrypsin, Transferrin, Retinol Binding Protein, alpha-1-acid glycoprotein, beta-2-Glycoprotein, and IgG, IgA, IgM, and their fragments;
- (b) separating excess unbound glycoprotein or glycopeptide from said fluid sample after reacting said glycoprotein or glycopeptide with bacteria in (a);
- (c) measuring the amount of said glycoprotein or glycopeptide remaining after separating said excess unbound glycoprotein or glycopeptide in (b) by detecting a label added to said glycoprotein or glycopeptide before binding with bacteria or adding a label after separating said excess unbound glycoprotein or glycopeptide;
- and
- (d) determining the bacteria context of said sample as related to the amount of said label measured in (c).
Dependencies are inferred from the claim text.