Compositions for binding to assay substrata and methods of using
AssigneeLyotropic Therapeutics, Inc.
InventorsDavid Anderson, Richard Saul
Life of this patent
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Abstract
Compositions and methods for binding to assay substrata in a stable and protective manner, thereby enhancing assay performance, are provided. The compositions comprise lyotropic materials (for example, lyotropic liquid and/or liquid crystalline materials) and may contain macromolecular standards, markers or capture compounds. The compositions are capable of binding to assay substrata such as that of chips that are employed for MALDI and SELDI mass spectroscopy analyses and plates that are used for ELISA type assays.
Claims92
A calibration method for use in assays, comprising the steps of:
- applying to a surface of a substrate a composition comprising lyotropic liquid or liquid crystalline material in which is incorporated one or more marker molecules, said composition binding to the surface of said substrate;
- and
- using data which is derived from said one or more marker molecules as a calibration standard.
A composition or kit used for calibration in an assay, comprising:
- at least a first particle formed from a lyotropic liquid or liquid crystalline material and having a first protein or peptide marker molecule;
- at least a second particle formed from a lyotropic liquid or liquid crystalline material and having a second protein or peptide marker molecule, wherein said second protein or peptide marker molecule is different from said first protein or peptide marker molecule,
- wherein each of said at least a first particle and said at least a second particle bind directly to a surface of a substrate suitable for use in an assay.
A method of performing an assay for a molecule of interest in a sample, comprising the steps of
- combining a sample with a composition of lyotropic liquid or liquid crystalline material
- binding said composition of lyotropic liquid or liquid crystalline material to a substrate;
- and
- measuring for one or more molecules of interest on said substrate.
A method of performing an assay, comprising the steps of:
- combining a sample with a composition of lyotropic liquid or liquid crystalline material which has incorporated therein one or more capture molecules;
- allowing one or more analyte molecules in said sample to bind with said one or more capture molecules in said composition of lyotropic liquid or liquid crystalline material;
- binding said composition of lyotropic liquid or liquid crystalline material to a substrate;
- and
- measuring the analyte molecules bound to said capture molecules.
A composition used for calibration in an assay, comprising
- a plurality of particles formed from lyotropic liquid or liquid crystalline material, which bind directly to a surface of a substrate, each of said plurality of particles having at least two different marker molecules present in the particle.
A method for performing a laser desorption ionization assay, comprising the steps of:
- binding a lyotropic liquid or liquid crystalline material to a surface or substrate on which a sample is or will be deposited;
- coating a layer of said lyotropic liquid or liquid crystalline material and said sample with a chemical which crystallizes in situ to form an energy absorbing matrix;
- and
- measuring one or more compounds of interest in said sample after said binding and coating steps using laser desorption ionization.
A method of preventing non-specific binding during an assay that uses a solid support or substrate, comprising the steps of:
- binding one or more capture molecules to a surface of said support or substrate;
- binding lyotropic liquid or liquid crystal material to said surface of said support or substrate at locations on said surface where capture molecules are not bound., whereby samples exposed to said support or substrate are presented with one or more regions for enabling specific binding said one or more capture molecules and are blocked from non-specific binding to said surface of said support or substrate by said lyotropic liquid or liquid crystal material.
A particle, comprising:
- a lyotropic liquid or liquid crystalline matrix;
- a first coating on a surface of said lyotropic liquid or lyotropic liquid crystalline material;
- and
- a second coating on a surface of said first coating, said second coating being different chemically and/or physically from said first coating.
A particle, comprising:
- a lyotropic liquid or liquid crystalline matrix;
- and
- a constituent associated with said lyotropic liquid or liquid crystalline matrix which, upon activation by a change in pH, temperature, or other physical or chemical condition, forms a coating on said lyotropic liquid or liquid crystalline matrix, said coating causing said particle to bind directly to a surface of a substrate.
Dependencies are inferred from the claim text.