Automatic large-scale screening of cells secreting monoclonal antibodies
AssigneeMonoclonal Antibobdies Therapeutics
InventorsJean Kadouche, Marc Lechuga, Albert Roseto
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Abstract
The invention relates to an automatic method for the large-scale in vitro screening of cells, secreting at least one specific monoclonal antibody with affinity for a compound of interest, comprising: (10) distribution of antibody-producing cells on at least one culture plate, (12) culturing of said cells, (14) iterative screening of said cells for the secretion of antibodies with cloning of the cells secreting at least one antibody interacting with the compound of interest and (16) selection of at least one cell secreting a specific monoclonal antibody with affinity for said compound of interest.
Claims48
An automated method for the large-scale in vitro screening of cells secreting at least one specific monoclonal antibody with affinity for a compound of interest, said method comprising at least the following steps:
- (10) distribution of antibody-producing cells in at least one well of at least one culture plate;
- (12) culturing said cells under conditions allowing their growth, with concomitant detection of cellular growth and of the quality of the cultures;
- (14) iterative screening of said cells for the secretion of antibodies, with cloning of the cells secreting at least one antibody interacting with said compound of interest;
- and
- (16) selection of at least one cell secreting one specific monoclonal antibody with affinity for said compound of interest.
(canceled)
Dependencies are inferred from the claim text.