Scintillation proximity test
AssigneeGe Healthcare Limited
InventorRobert Jessop
Life of this patent
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Abstract
The invention concerns scintillation proximity assays performed in multiwell plates where a charge coupled device is used to image the wells. Conventional phosphors emit blue light (350-450 nm) which is absorbed by yellow or brown assay components. This problem is addressed by the use of phosphors that emit radiation of longer wavelength (480-900 nm).
Claims10
In a scintillation proximity test which uses a phosphor for detection, the improvement comprising using a phosphor that has an emission maximum of 480 nm-900 nm, and of a such that a cooled charge coupled device for detecting radiation emitted by the phosphor is utilized as part of the detection system.
A method of performing a scintillation proximity test comprising:
- providing a solid surface comprising a phosphor in a fluid medium,
- causing a radiolabelled reagent to become divided into two fractions one bound to the solid surface and the other in the fluid medium, and
- detecting the fraction of the radiolabelled reagent bound to the solid surface,
- characterised by using a phosphor that has an emission maximum of 480 nm-900 nm and a cooled charge coupled device for detecting radiation emitted by the phosphor.
Dependencies are inferred from the claim text.