Hyaluronan receptor for endocytosis
OwnerPaul Weigel, Janet Weigel +1inventors
InventorsPaul Weigel, Janet Weigel, Bin Zhou
Life of this patent
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Abstract
A purified nucleic acid segment encoding a functionally active hyaluronan receptor for endocytosis (HARE) or an active peptide fragment thereof, and methods for producing functionally active HARE or an active peptide fragment thereof therefrom, wherein the functionally active HARE or an active peptide fragment thereof is able to specifically bind HA, chondroitin and chondroitin sulfate.
Claims24
A purified antibody that binds specifically to a HARE protein.
A purified antibody that selectively binds to an epitope of a HARE protein.
A purified antibody that binds specifically to a HARE protein and inhibits the binding of at least one of HA, chondroitin and chondroitin sulfate to the HARE protein.
A monoclonal antibody having the same epitope specificity as at least one of:
- (a) mAb-28, ATCC accession number PTA-5354;
- (b) mAb-30, ATCC accession number PTA-5355;
- (c) mAb-54, ATCC accession number PTA-5356;
- (d) mAb-159, ATCC accession number PTA-5358;
- (e) mAb-174, ATCC accession number PTA-5359;
- (f) mAb-235, ATCC accession number PTA-5359;
- and
- (g) mAb-467, ATCC accession number PTA-5361.
A method of making an antibody, comprising immunizing a non-human animal with an immunogenic fragment of a HARE protein.
A method of making a humanized monoclonal antibody, comprising:
- immunizing a non-human animal with an immunogenic fragment of a HARE protein to form a monoclonal antibody;
- and
- removing portions of the monoclonal antibody which may be antigenic in a human and substituting like portions of a human antibody therefor.
A method of making a monoclonal antibody, comprising the steps of:
- identifying a hybridoma cell that produces a monoclonal antibody specific for a HARE protein;
- and
- culturing the hybridoma cell under conditions that permit production of the monoclonal antibody.
A method of purifying a functionally active HARE protein wherein the HARE protein is able to specifically bind at least one of HA, chondroitin and chondroitin sulfate, comprising the steps of:
- providing a biological sample containing a HARE protein;
- providing an affinity matrix comprising:
- an antibody that selectively binds to an epitope of a HARE protein;
- and
- a solid support to which the antibody is bound;
- contacting the biological sample with the affinity matrix such that the HARE protein in the biological sample binds to the antibody of the affinity matrix;
- separating the HARE protein bound to the antibody of the affinity matrix from the remainder of the biological sample;
- and
- releasing the HARE protein from the antibody of the affinity matrix.
Dependencies are inferred from the claim text.