Polynucleotides encoding plant cysteine proteases
OwnerSaverio Falco, Edgar Cahoon +6inventors
InventorsSaverio Falco, Edgar Cahoon, Omolayo Famodu, Theodore Klein, Emil Orozco, J. Rafalski, Jennie Shen, Hajime Sakai
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a proteinase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the proteinase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the proteinase in a transformed host cell.
Claims15
An isolated polynucleotide comprising a first nucleotide sequence encoding a polypeptide of at least 40 amino acids that has at least 85% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:2, 4, 6, 30, 32, and 34,
- or a second nucleotide sequence comprising the complement of the first nucleotide sequence.
2-30. (canceled)
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having cysteine protease activity, wherein the polypeptide has an amino acid sequence of at least 80% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO:24, or
- (b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.
Dependencies are inferred from the claim text.