Thermus brockianus nucleic acid polymerases
AssigneeApplera Corporation
InventorsJames Rozzelle, Elena Bolchakova
Life of this patent
Estimating the term…
Abstract
The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides.
Claims14
1-31. (canceled)
An isolated nucleic acid polymerase comprising any one of amino acid sequences SEQ ID NO:9-16.
An isolated nucleic acid polymerase comprising any one of amino acid sequences SEQ ID NO:9-49.
An isolated nucleic acid polymerase comprising any one of amino acid sequences SEQ ID NO:9-10 having a mutation that decreases 5-3′ exonuclease activity.
An isolated nucleic acid polymerase comprising any one of amino acid sequences SEQ ID NO:9-10 having a mutation that reduces discrimination against dideoxynucleotide triphosphates.
A kit comprising a container containing a nucleic acid polymerase, wherein the nucleic acid polymerase comprises any one of amino acid sequences SEQ ID NO:9-9.
A kit comprising a container containing a nucleic acid polymerase, wherein the nucleic acid polymerase comprises any one of amino acid sequences SEQ ID NO:9-16.
42-43. (canceled)
A nucleic acid polymerase made by the method of claim 42.
45-54. (canceled)
Dependencies are inferred from the claim text.