Chemical amplification for the synthesis of patterned arrays
AssigneeAffymetrix, Inc.
InventorsJody Beecher, Martin Goldberg, Glenn McGall
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Abstract
Radiation-activated catalysts (RACs), autocatalytic reactions, and protective groups are employed to achieve a highly sensitive, high resolution, radiation directed combinatorial synthesis of pattern arrays of diverse polymers. When irradiated, RACs produce catalysts that can react with enhancers, such as those involved in autocatalytic reactions. The autocatalytic reactions produce at least one product that removes protecting groups from synthesis intermediates. This invention has a wide variety of applications and is particularly useful for the solid phase combinatorial synthesis of polymers.
Claims17
1-51. (canceled)
A method for removing an acid labile protecting group from a synthesis intermediate comprising the steps of
- a) forming a surface comprising a photosensitive active catalyst wherein said photosensitive active catalyst produces acid upon exposure to light of a pre-determined wavelength;
- b) irradiating at least a part of said surface to remove said protecting group.
A method for synthesizing an array of diverse polymers on a substrate comprising the steps of:
- a) providing a substrate comprising a reactive functional group protected by an acid sensitive protecting group;
- b) forming a surface on said substrate comprising a photosensitive active catalyst wherein said photosensitive active catalyst produces acid upon exposure to light of a pre-determined wavelength;
- c) irradiating at least a part of said surface to remove said protecting group and expose said reactive functional group;
- d) washing said substrate to remove said surface;
- e) contacting said substrate with a synthesis intermediate to react with said exposed functional group;
- and
- f) repeating steps a-e to form the desired array.
Dependencies are inferred from the claim text.